ICAD (DFFA) (NM_004401) Human 3' UTR Clone

CAT#: SC211368

3`UTR clone of DNA fragmentation factor 45kDa alpha polypeptide (DFFA) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DFFA
• Synonyms: DFF-45; DFF1; ICAD
• ACCN: NM_004401
• Insert Size: 969 bp
• Sequence Data:
  Insert Sequence

  >SC211368 3'UTR clone of NM_004401
  The sequence shown below is from the reference sequence of NM_004401. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATCCTAAGCGAGCCAGACAGGATCCCACATAGCAGCAGCGGGAAGTGTGCCAAGGAAGCTCTGTGGCGTT
  GTGTTATTGGTAGACACCCTCAGCCTCATCATTTGACTACCTATGTACTACTCTACCCCCTGCCTTAGAG
  CACCTTCCAGAGAAGCTATTCCAGGTCTCAACATACGCCGTTCCACCAATTTTTTTTTTAGCCCCACCAG
  CTTCAGGACTTCTGCCAATTTTGAATGATATAGCTGCACCAACAATATCCCGCCTCCTCTAATTACATAT
  GATGTTCTCTGTTCAAAAGTAATTGGCAGTGATTGGCCAGGCGCAGTGGCTCACGCCTGTAATCCCAGCA
  CTGGGAGGCCGAGGGGGGCGGATCGTGAAGTCAGGAGATCGAGACCATCCTGGCTAACATGGTGAAACCC
  TGTCTCTACTAAAAATACAAAAAAAATTAGCCAGCCATGGTGGCGGGCGCCTGTAATCCCAGCTACTTGG
  GAGGCTGAGGCAGGAGAATGGCATGAACCTGGGAGGCAGAGCTTGCAGTGAGCTGAGATTGCGCCACTGC
  ACTCCAGCCTGGGCAACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAGTAATTGGCAGTGACT
  ATGGGCGCACTGCCTAACATTTAGCCCTGCCCCATATGGAACATGTTAAAAAAAAAAAAAGCCAGGCCGA
  GCGTGTTGGCTCACGCTTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGA
  TCGAGACCATCCTGGCTAACACAGTGAAACGTGTTTTTACTAAAAGTACAAAAAACTAGCTGGGCGTGGT
  GGCAGGAGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAACCGGGGAGGCAGAA
  GTTGCAGTGAGCCGAGATTATGCCACTGTACTCCAGCCTGGGTGACAGAGTGACACTCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004401.2
• Summary: 'Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 1676