PGAM1 (NM_002629) Human 3' UTR Clone

CAT#: SC211372

3`UTR clone of phosphoglycerate mutase 1 (brain) (PGAM1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PGAM1
• Synonyms: HEL-S-35; PGAM-B; PGAMA
• ACCN: NM_002629
• Insert Size: 963 bp
• Sequence Data:
  Insert Sequence

  >SC211372 3'UTR clone of NM_002629
  The sequence shown below is from the reference sequence of NM_002629. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCAAAGCCATGGAAGCTGTGGCTGCCCAGGGCAAGGCCAAGAAGTGAAGGCCGGCGGGGAGGATACTGTC
  CCCAGGAGCACCCTCCCTGCCCGTCTTGTCCCTCTGCCCCTCCCACCTGCACATGTCACACTGACCACAT
  CTGTAGACATCTTGAGTTGTAGCTGCAGACGGGGACCAGTGGCTCCCATTTTCATTTTAGCCATTTTGTC
  GCCTGCACCCACTCCCTTCATACAATCTAGTCAGAATAGCAGTTCTAGAGCACAGGTTCTCAGTCTAAGC
  TATGGAAAAGCTCCCCTTATCCAACAGAGTTTAAAAGTAGTGACTTGGGTTTTTGCGAGTGCTTTGTTTA
  CTAAGGACTTTGGGGAGGAACCATGCTAAGCCATGACCAGTGAGGAGAAGCAACAGAGCCTGTCTGTCCC
  CATGAGCGGAGTCTGTCCTCTGCTCTTCTGCAGTCAGGTCACTGCCTACTGCCTGGGGGCTCTAGTCATT
  CCAGTGGAAGACGAATGTAACCTGCGTGGTGATGTGACAACTGTTTCCTCCCTGACCCCAGAGGATCTGG
  CTCTAGGTTGGGATCAATCCTGAATTTCGTTATGTGTTAATTTACTTTTATTAAAAAAGTATAGTATATA
  TAATACAAAACAATAACCCTTCTGGGGTTTCTTGTGGCGGTTGAAATAGTCCCACATGTGGTCATCAGAA
  AATAAGCCATTCCTCATACCAATATAGGATCAGCTCCTTGACCTCTGAGGGGCAGGAGTGCTTCCTGGTG
  TGTGTATTAGAATCCCTTCCTGCCTTGTTTCATGGCAGTGAAATGCCTCTTGGTCCTGTCCAAGTGTATC
  TTTCACTGATTTCTGAATCATGTTCTAGTTGCTTGACCCTGCCACATGGGTCCAGTGTTCATCTGAGCAT
  AACTGTACTAAATCCTTTTTCCATATCAGTATAATAAAGGAGTGATGTGCAAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002629.2
• Summary: 'The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]'
• Locus ID: 5223