RFC5 (NM_007370) Human 3' UTR Clone

CAT#: SC211458

3`UTR clone of replication factor C (activator 1) 5 36.5kDa (RFC5) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RFC5
• Synonyms: RFC36
• ACCN: NM_007370
• Insert Size: 971 bp
• Sequence Data:
  Insert Sequence

  >SC211458 3'UTR clone of NM_007370
  The sequence shown below is from the reference sequence of NM_007370. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCAAGTCACCAGAGACCTGATTGTTGCAGAGGCCTAGATGCTCTGAGGGCCATTCACAATTCTCAGGGCT
  CAGCAGTGATGGGAGAACAGAGGACAGTTCCAGGATAAACTGCTGCCTGGGGCTGTGGGATGAATCAGTC
  ACCCCGAATCTTGGAAAAACCCCCTTCCAGGAGAGGATGGGCAGGCATTTAAAAAGTACCATTTTTGTGG
  TTGTTTGGAGCAGGGATGTACAAAATAATTTTAATGTATTAACTCATACTGCCTGTCTTTTATAGGGGAA
  AAAAATAACCTTTTTTATTTTAAAGTTATAAGGTTTTTACCTTTTAGTTGCTTGGATGACAGGGAATTAG
  CCTACCCCATTTTGGTCTGGAACAGAAGACTTTCAAATTTAATATGGTCCAAGTGTCTTCCTACTCAAGG
  TAAACATTATCTCCAAAATTACATTTATGATTCTAATATTTGGCATTGTGTCTGTATCTAATTTAAACAG
  ATGTTAATGGACGTCTGGCCGAAACTATTATACTTTTATAAGATGAGCTGAATCCTCTTACTTTAAAAAC
  TGGTCTTTTTATTTACCCTCTGTGGTAGAAGAGTCACCAGCAGGTTCCAAATTGATGTGTATGATAGGAA
  AAAAACCTTAATTTTAAATATAATATAGAGCCTTAAACTATGCCACTGGGTGGCAGAGGCTGTATAAAAC
  GCACTTGTTTTCATGCAGGAGCGGGGCAAGTAAGGTTGAGCCTGACTGTAAACCTAGAACTGCGGAAGGA
  CTGGGACTTTTGTACAAATTTCACATTTATTTTACAGCAATCATGCTGCATTTGTGTTAGTGTTCTTTAT
  AATAAAAAACAAAGCAGCCTCCTGGCTGGCTGTGTTACAAGTGACTCTTCCTTAGACAGCTAGAAAGATG
  CTGACTTTGTCAAACTTGCATTTATATGAAAACCTTCTGTCTCTAATATCACAGAATAAAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_007370.4
• Summary: 'This gene encodes the smallest subunit of the replication factor C complex, which consists of five distinct subunits (140, 40, 38, 37, and 36 kDa) and is required for DNA replication. This subunit interacts with the C-terminal region of proliferating cell nuclear antigen and is required to open and load proliferating cell nuclear antigen onto DNA during S phase. It is a member of the AAA+ (ATPases associated with various cellular activities) ATPase family and forms a core complex with the 38 and 40 kDa subunits that possesses DNA-dependent ATPase activity. A related pseudogene has been identified on chromosome 9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]'
• Locus ID: 5985