RASGRP 4 (RASGRP4) (NM_001146204) Human 3' UTR Clone

CAT#: SC211703

3`UTR clone of RAS guanyl releasing protein 4 (RASGRP4) transcript variant d for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RASGRP4
• Synonyms: guanyl nucleotide releasing protein 4; RAS guanyl releasing protein 4
• ACCN: NM_001146204
• Insert Size: 982
• Sequence Data:
  Insert Sequence

  >SC211703 3'UTR clone of NM_001146204
  The sequence shown below is from the reference sequence of NM_001146204. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAACTGCATCCTCCAAGCTGGATTCCTAGACATCTCTTGGTCTCCTCTCTTCCTCACTCCCTTCCCCCAG
  TCAGTCCTGAGTCCTGCCGTCAGACTCTGGCAGGGCTCCCAGAGGTAGGCCTCCAGAGGCATCCGCCTTC
  CCATCCACACTGCCTTTAGTGGTACGTCCGTCATCTTTTTCCCTGGAAGTGACTTTCCTCTTTTGCATCC
  TGGTGGATGCTAATCCTGCTCCCTTTCCCTGCACTTAGTATCTGTGCGGCTGTGTGTTGGATACATTTAG
  ACTCAGGCCTCATTCTACCAACTCCCTACATCTCCATTTCTTTTTTTTTTTGAGACAGAGTCTTGCTCTG
  TCACCCAGGCTGGAGTGCAATGATGCAATCTCAGCTCACTGCAACTTCCGCCTCCTGGGTTCAAGTGATT
  CTTCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCACGCGCCACCATGCCCGGCTAATTTTTGTATT
  TTTAGTAGAGACGGGGTTTCCCCATGTTGGCCAAGATGGTCTCAAACTCCTGACCTCATGATCCACCAGC
  CTCAGCCTCCCAAAGTGCTGGGAATACAGGCGTGAGCCACCACGCCCAGCTCCTGTTTTTCTGCATAGAA
  TGTGCTCCCTCTGACAGCCTATACTTTTTCCCTCTTTATCTCGTTGAACGTCTGTCTTTCCTCAACAGGA
  GCATCAACTCCAGGAGAACAGGGATTTCTATCAATGTCATTCACTGCTGCATCCCCAGTGCCTAGAACAG
  GGCTGGCAGGTGGTAGGCGCCTGACAGATGCTTGTCTGATGCCTGAATGTCTCCTTACCCATGCCCACGG
  CACAGGATAGATGTGCTATAGGGCAAAGAACTTTGAGGGTCGAGCAGCAGGGGCCCATTCAGTCCCAGGG
  AGCAGAGACCCCCCCAACCCCTTCACAAACCCCAACACCCCTGACTTGGCCCCCACAGAGAGAGGTCCTA
  CA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001146204.1
• Summary: The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
• Locus ID: 115727