MEK3 (MAP2K3) (NM_145109) Human 3' UTR Clone

CAT#: SC211773

3`UTR clone of mitogen-activated protein kinase kinase 3 (MAP2K3) transcript variant B for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MAP2K3
• Synonyms: MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2
• ACCN: NM_145109
• Insert Size: 1022 bp
• Sequence Data:
  Insert Sequence

  >SC211773 3'UTR clone of NM_145109
  The sequence shown below is from the reference sequence of NM_145109. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACATTGCTGCCTTCGTGAAGGAGATCCTGGGAGAAGACTCATAGGGGCTGGGCCTCGGACCCCACTCCG
  GCCCTCCAGAGCCCCACAGCCCCATCTGCGGGGGCAGTGCTCACCCACACCATAAGCTACTGCCATCCTG
  GCCCAGGGCATCTGGGAGGAACCGAGGGGGCTGCTCCCACCTGGCTCTGTGGCGAGCCATTTGTCCCAAG
  TGCCAAAGAAGCAGACCATTGGGGCTCCCAGCCAGGCCCTTGTCGGCCCCACCAGTGCCTCTCCCTGCTG
  CTCCTAGGACCCGTCTCCAGCTGCTGAGATCCTGGACTGAGGGGGCCTGGATGCCCCCTGTGGATGCTGC
  TGCCCCTGCACAGCAGGCTGCCAGTGCCTGGGTGGATGGGCCACCGCCTTGCCCAGCCTGGATGCCATCC
  AAGTTGTATATTTTTTTAATCTCTCGACTGAATGGACTTTGCACACTTTGGCCCAGGGTGGCCACACCTC
  TATCCCGGCTTTGGTGCGGGGTACACAAGAGGGGATGAGTTGTGTGAATACCCCAAGACTCCCATGAGGG
  AGATGCCATGAGCCGCCCAAGGCCTTCCCCTGGCACTGGCAAACAGGGCCTCTGCGGAGCACACTGGCTC
  ACCCAGTCCTGCCCGCCACCGTTATCGGTGTCATTCACCTTTCGTGTTTTTTTTAATTTATCCTCTGTTG
  ATTTTTTCTTTTGCTTTATGGGTTTGGCTTGTTTTTCTTGCATGGTTTGGAGCTGATCGCTTCTCCCCCA
  CCCCCTAGGGTACCAGCAGGCAGAGCCTTGCCCTCTGCTCAGGCTGGGGTCCAGTGGGAGGGGCCCAAGA
  TCTCTGCTCAGAGAAGTGCAGGGGGAGCCTTCCAGCTCACTCTCCCTGAGGACTGGCTTGACAGGGGCTA
  TGGGTTTGCTTTGGTGTTGTTTTTAAAAAAAGAAAATATATTTTTTTGAAAAAACGACTGCCCATCCCGG
  GTCCTTTCCCTGATGGGTTGGGGCAGTTACCTGGTTGCTGTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_145109.2
• Summary: 'The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 5606