Plectin (PLEC) (NM_201381) Human 3' UTR Clone

CAT#: SC211888

3`UTR clone of plectin 1 intermediate filament binding protein 500kDa (PLEC1) transcript variant 7 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PLEC
• Synonyms: EBS1; EBSMD; EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN; PLEC1; PLEC1b; PLTN
• ACCN: NM_201381
• Insert Size: 1031 bp
• Sequence Data:
  Insert Sequence

  >SC211888 3'UTR clone of NM_201381
  The sequence shown below is from the reference sequence of NM_201381. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCCCTGAGTCTGCCGTGGCCTGAGGCTGCCTGCGCCCACCCCGCTCTGCATGCGGCCCAGCCCGGCTCCC
  ACCGAGGCGCGGGGGCCGTTTTCAACGCTTAAAGGTGTCTTCCTCCCAAGTGGTGCCTAAAGTTTAACCA
  AAAAGACCAGACTAATATATTAATATATATCTGCTGTCCAGACAGCCTGTATCTTGGGGGACAGGGCTGG
  CCCAGCCCTGCTGGCCGCCTCACCCCCTCGGGTCTCCTCACTCCCTTCTACCTGCCACTCACACAGCCAG
  GTGCCTTGGAGGGTCCCAAGCTGGGCCCCAGCCCACCCTCCTGTCTTCCCAGGGTAGCCCGCCTGCCAGT
  CCTAGCTGCACAGGGCAGCTGGGCCCAACCCTGTCTGTAGAGGGCCCTGGTGTTTCTAGCACTGGCCTGC
  ACGGTGGGCCTTGCTGGGGACGGGGGGCCCCAGTCAGCCTCTCTCCCAGTCTACCCAGAGAAGCCCCTTC
  CCCATGGGAAGACGAGGCCCTCGGGCCCAGCCCCCACAGTGCTGTCTGATCTGTGCTTTCCAGCTCACCC
  CCCACACTCACTCCTGAGACCCCTGGCCTCCGGCGTCAGCCTCCAGCCTCTGTTCCCCTAGTAAGTGCCT
  TCCATGTCGGCCTCTAACCCCAGGCCCCGAGGACCCAGACCCAGTGGGGAGGCGGACGTTCCAGCCGGCA
  TGGCTGGGAACTGCAGACCTGTCCTCCTGGTGGGTCCAGGGGCCCCTCCAGCTTGTGGAGCCCCACACTG
  GGGTGCCGCCTGCCCGTCTCTCTCCCATGGAGCCCCAGCCCCCTTTGGGCCCAGGGACACCAGCCAGGCT
  CTGTGCTGACCCTCCTGTTGCACCCAGCCCTGGTCTCAGCAGCGACCACCCCTGCCTCCACCCTCTGAGC
  TTTGCATGTTCCACTAACCCCGGGCGGGTGGCAGGTGGAGGTGTCAGGCTGCTGGCGCCTCTGCAAGGGC
  AGAACACTAACCTGACCGTGGGCGGGGCCTTGCGGTATCCGCCCCCAATAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_201381.1
• Summary: 'Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes (reviewed in PMID: 9701547, 11854008, and 17499243). Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse and rat) and the gene product had been referred to as "hemidesmosomal protein 1" or "plectin 1, intermediate filament binding 500kDa". These names were superseded by plectin. The plectin gene locus in mouse on chromosome 15 has been analyzed in detail (PMID: 10556294, 14559777), revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms. Eleven exons (1-1j) have been identified that alternatively splice directly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD). Three additional exons (-1, 0a, and 0) splice into an alternative first coding exon (1c), and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8). Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat (PMID: 14672974); exons 1i, 1j and 1h have not been confirmed in human. Furthermore, isoforms lacking the central rod domain encoded by exon 31 have been detected in mouse (PMID:10556294), rat (PMID: 9177781), and human (PMID: 11441066, 1'
• Locus ID: 5339