SIAH1 (NM_003031) Human 3' UTR Clone

CAT#: SC211989

3`UTR clone of seven in absentia homolog 1 (Drosophila) (SIAH1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SIAH1
• Synonyms: SIAH1A
• ACCN: NM_003031
• Insert Size: 1082 bp
• Sequence Data:
  Insert Sequence

  >SC211989 3'UTR clone of NM_003031
  The sequence shown below is from the reference sequence of NM_003031. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAAAATGGCAATTTAGGCATCAATGTAACTATTTCCATGTGTTGAAATGGCAATCAAACATTTTCTGGCC
  AGTGTTTAAAACTTCAGTTTCACAGAAAATAAGGCACCCATCTGTCTGCCAACCTAAAACTCTTTCGGTA
  GGTGGAAGCTAGACACATGAAGGTAAATAAAAAGAAAGGCTGTTAAATACAGGAAACAGTTGCATGTAGT
  AACACTAATATATTTAAAAATAAGTCAACAGTAAACCACTGAAAAAATATATGTATATACACCCAAGATG
  GGCATCTTTTGTATTAAGAAAGGAAGCATTGTAAAATAATTCTGAGTTTTGTGTTTGTTGTAGATTGATT
  GTATTGTTGAAAAAGTTTGTTTTTGCGTGGGAGTGTGTGCCTGCGTGGGTGTGTGCGTGTTTGGGTTTTT
  TTCCTTTAACTGACAAGCCATCTTGAGTGGTCATGGGCCACTGCTTTTCCCTTTGTGAGTCAATACATAG
  TGCTGCTGTGTGCTTTTTTTGTGTGTATTTGCTAATTTTTATTAATTTTAGTTTTTCATTAAATAAATTT
  GACTTTTCTGTAATTCAGGTTTTTCCTTTTTTTGTACCATTTTAAAGTTAGTATCTTTTGATATGCATAT
  TTGTTTATGGTAAAAAATTTATAACGTGTTCAATATTTTCTTTTCCCCCATTAATCAGTTCATTAGAAAT
  ATTTTAAAATCAGCTATTTTGTGAAGCCATGAGTTCCAGAAAGTAAAGGTGACATCGGAAAAATAATCAA
  AAGCTATTTAAAGCATCTATAAGGTGCTCTCTTTCTGTCTTCTACAGATGAGTCACACCTTTGAGCTTAA
  TCTTTGAAAGGTTAGAGAATAAATTGATTTTTATAAATACTGCAAATCAGGCTTTTGTTTCCTTTTTCAG
  ATATCTTGGACAAATCACATATTTTAAAATTTGTTCTTGTATTTATTGGTTTTGCAGAAGAAGGCATCGT
  CATGCACAGTATTTGTAATTAAAAGCAAATCATTTGTTTAAAAAGGCAGTTTGCAAAAAATGTTTTTGGT
  CTTTTATAATTCTCATTAAAAGAATATCTGGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003031.3
• Summary: 'This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]'
• Locus ID: 6477