hnRNP F (HNRNPF) (NM_004966) Human 3' UTR Clone

CAT#: SC211996

3`UTR clone of heterogeneous nuclear ribonucleoprotein F (HNRNPF) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HNRNPF
• Synonyms: HNRPF; mcs94-1; OK/SW-cl.23
• ACCN: NM_004966
• Insert Size: 1020 bp
• Sequence Data:
  Insert Sequence

  >SC211996 3'UTR clone of NM_004966
  The sequence shown below is from the reference sequence of NM_004966. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAACAGCATGGGTGGCTATGACTAGTTTTGTTAGGAACATTTGAGTTACTTCAATCATTTTCACAGGCAG
  CCAACAAGCAATTAAGAGCAGTTATAATAGAGGAAGCTGGGGGACCCATTTTGCACCATGAGTTTGTGAA
  AAATCTGGATTAAAAAATTACCTCTTCAGTGTTTTCTCATGCAAAATTTTCTTCTAGCATGTGATAATGA
  GTAAACTAAAACTATTTTCAGCTTTTCTCAATTAACATTTTGGTAGTATACTTCAGAGTGATGTTATCTA
  AGTTTAAGTAGTTTAAGTATGTTAAATGTGGATCTTTTACACCACATCACAGTGAACACACTGGGGAGAC
  GTGCTTTTTTGGAAAACTCAAAGGTGCTAGCTCCCTGATTCAAAGAAATATTTCTCATGTTTGTTCATTC
  TAGTTTATATTTTCATTTAAAATCCTTTAGGTTAAGTTTAAGCTTTTTAAAAGTTAGTTTTGAGAATTGA
  GACACAATACTAATACTGTAGGAATTGGTGAGGCCTTGACTTAAAACTTTCTTTGTACTGTGATTTCCTT
  TTGGGTGTATTTTGCTAAGTGAAACTTGTTAAATTTTTTGTTAACTAAATTTTTTTCTTAAAATAAAGAC
  TTTTTCACAATGACTGGCACAGATTACTCAGCAAAAGATAGCAAAACGGGTGGTTGAAGATAATTCATTT
  TAATCGTAATGTATTTTAGTGTGAATTTAAAAATTTCATACATCAAATCTATGATCTCCCTTATATTCTT
  ATGATGAGGCTAAATAAAAGTCTAATAAAAATGTTAAATATGTGAATGGTGGAAATGGTGACTAGCAGCA
  CACATTCTGGGAAGCATCAAATAGACACACGGCCCCAGCCACCTGCAACTTATGTGCACTGTTGTAAACC
  ATTCAGAATTTTCCTGCTAGGCCCTTGATGCTGGAGTCACATCTGTTGATAGCTGGAGAACTTTAGTTTC
  AAGTACTACATTGTGAAAGCAATTATTTTGTTTCTAGTTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004966.3
• Summary: 'This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]'
• Locus ID: 3185