RASA1 (NM_022650) Human 3' UTR Clone

CAT#: SC212042

3`UTR clone of RAS p21 protein activator (GTPase activating protein) 1 (RASA1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RASA1
• Synonyms: CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP; p120RASGAP; PKWS; RASA; RASGAP
• ACCN: NM_022650
• Insert Size: 1051 bp
• Sequence Data:
  Insert Sequence

  >SC212042 3'UTR clone of NM_022650
  The sequence shown below is from the reference sequence of NM_022650. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AACCAATGATGTCAGGTAGCAGCCTTCGCCCCAGTGTTCTGCATGGATTCAGCATGTCCAACATGGTAAT
  TCACTTCAGTTTAATGTCTCCTTTGCTCTTGCCAAAAAATAGCACACTTTTCCACATTCCAGTGATGTGT
  GAGCTATGCAAACAAAATCCAAGATTCTGCTGGTGAATAACTATGCCAGCAACCTTGTAAGCTATCTGTG
  CAGGATATTTGCACTATTTCCACATGGAATCAATCTTTAACAACCTCTGAGCCTTGGTGTACAGACCACC
  TTTCACAAAACGAAATGCTATGACTGTATCTTGATATCTCGAACTTTCAAAATATATTTTCAGTACACCC
  AGTTGCCAAAGTTTTGCTGTCTCTTAGAGAAAGAACTATGAAATCAACTGACAAGAAACACATTCTTATT
  GACAATTGTGTATAACTGGATTGCAGACTGTTCTTACTGTAACTACTTCCTGATTAGGAATATGACCATT
  TGACTGTTCAATGATTATTTGTATTTACAGTTTCCAGAGTTTGTCATTATAATAGGAACAATCTTTGCTG
  TATACTTTTAAAAAATACTCTGCTATTTCTCTTGCTGGAACTGTTGAAAGAAAATATATAGAATGATCTA
  TTGCTCATCAGCTTTATTTTTTAAACATACGACTTATTTTGTTGAAATTGTCAAAGACTGTATTTAGATC
  TCATAATGCTTTGTTAAATGTTTACAAGTAAATAGTTTGAATTCAGTAAATATTATTGGTTGTTGTATTG
  ATCAATGCATGTTACCCATTCAACCATTTTATAGACTACCAATTTCTTTTATGTTAACTAGAATGCTTTT
  GTTAAAAGTTATTTGTTCATTATTTGTGCTACCCCTTTGATTATGCAGACAACCTCATCAGCTGCCTAAC
  TTATCCATCTTTGAACTTCTGACTACTTGTTGTATCTGCTGGATATTTAGTTCAACTGTATAGTTTTATT
  TACTTCTGTATGTGTATTTTTGTGAAGTATTCACAAAGGTTAAGTTAAAATAAAACCAAGGGATATCTTG
  C
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_022650.1
• Summary: 'The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]'
• Locus ID: 5921