DNA polymerase alpha (POLA1) (NM_016937) Human 3' UTR Clone

CAT#: SC212078

3`UTR clone of polymerase (DNA directed) alpha 1 catalytic subunit (POLA1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: POLA1
• Synonyms: NSX; p180; POLA
• ACCN: NM_016937
• Insert Size: 1050 bp
• Sequence Data:
  Insert Sequence

  >SC212078 3'UTR clone of NM_016937
  The sequence shown below is from the reference sequence of NM_016937. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGAGCAAACTCTTCGCTGGTTGTGCCGTGAAATCCTAAGGGAATCCCAGGAGTAACCAAGGAGGGGGTA
  GTTGAAAAATCCCAGCTTCCTCTGTGCCTCCACTCTGGCCCTAAATGCTCCTCCAGCATCTGTTTCTCCC
  TTGGGACTGTGTCTCATGTTTGTGTGAATGTAGACCAGGAAAGGGGGCTGCAAAAATGTTGAGTCTAATG
  TTCGTAAGCATCATAGAAATTCCTGTCTTCATATTAAGATGTACTGCTTTAAAACACAACTCCAGAGCCC
  CTCCCCAAGCTCCCCTCCCCAAGCTCCTGAAGACCCGGTTTCTGAGGGAGGGAAATTGCTACTTGGATTG
  AGAGTAGCTGGAATGTAAGTGACCCCAGGCTTTGCCTCAGGGCCTTTAGCCTATGTCCCCCCCACATAAA
  GAGAGCTTCTCAGAGCCTGACTGAAGAGCTGACGTTTTGCTTTTTCATATGCCAATTAAACCCGGTCTAA
  ATCCAAATGCTTCTCCAGCCATCCAGGAGTGGCTGTCCTTTTCAGTCTTGTCTTTTATATAGGTAGCTGA
  GGGGGAAGATTTAGAAGCCTTGCACTCACTAAATAGATTAAACAGAGCAGGCTTGTTTGTTGAATTGCTC
  CAAAGTCCAACAGACACACACTGAGCAGGTGTTTTACACTCACATTCCCTTTTTGCCCCTTAAATAGAAA
  GTGCAGGTAAAGGTTTATACAACAAGAAAGCACATTGAAAATAATTTGATACTCTAACAATCCATTAACA
  TGTGTAGGGGTTACGGTGAGGATCACTGTGTTGTATTCAGAAAAACGGGGAGAGGGATGCTTAATTGGCC
  CTGGCGCTTGCTATTTTTTTCTCATTTCTTCACAATAGGACCGTCTTTGGCAGCAGCAAAATGTATTTCA
  GTATGGCAGTCTTTCCTCTCTTACATTATTGGTAAGATTATACTAACAAAATGTTTCCCCTTGTACAATT
  ATGCTGTGTTTTTAAAAAACATTGACCTGTGTGTTTTTATAAAAGAAAAAGTATGTTGTGCCTTCTTCTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_016937.3
• Summary: 'This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010]'
• Locus ID: 5422