IKB epsilon (NFKBIE) (NM_004556) Human 3' UTR Clone

CAT#: SC212099

3`UTR clone of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon (NFKBIE) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NFKBIE
• Synonyms: IKBE
• ACCN: NM_004556
• Insert Size: 1067 bp
• Sequence Data:
  Insert Sequence

  >SC212099 3'UTR clone of NM_004556
  The sequence shown below is from the reference sequence of NM_004556. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGCTGTGTACCGACTGAAGCCAGGCAGGGTCTGGGATCCTCAGGGCTCCACCTCTCCATCTGGAAGCCG
  GAGCCATAACTGCTGCAGTTTGGGCCCAGGCTATGTGCTCTTCTGGTGCCCTAGGGACTGCTGTGGCCAG
  AGCCTGGGGCCAGCCAGTACAGTCCTGAGCCGAGGAGGAGGGACTGCAAGTGGAAGAGAGCCAGTCTGGA
  AGGAAGAGCTTTCCAGGTGGACAGGGCTTCTTGGAAGACCCCCAAAGCCCCAGGTATCCTGGGTGAAGCC
  TGTTTGCCTCTCTTGAAAATGGCAGGTGCTCTTGTTTTACCCATGTTGGGTCAGCCTGAAACTGCCAACC
  AGTAGGAAGCATGGACTCTCCTGAGTGAGAAGAGACTGAAATAGGAGCAAGCAGAACCCTGAGAGGTGTC
  CCATCTTATTGCTGTTGAGGACCCTGAAACACCGTTGTTTAAAGACTTCACACAGAAGGCTCTGAACTGA
  GCCACTGGGGAAGGGAAGTTTCAGTAACATGACACTAAAATGGCAGAGACGTTAAAAAAAGTTTTTCCCT
  TCTAGAGCTGTTTTGCGCGCATGCATGTCTGTGTGCATTGGGGCTTTTTAGACAGGCCTGCCCTGTGACT
  TTGTGGTAGAGGCAGAGAGAAGGAAATTGTCCCCTGAGCACGGTAGGGCCTTGCTGGGTGGGGTCAGAGG
  CCAGTAGTTCCAGGCCTTTCTCTGTGTCCAGCACAGACCCTTGTCCTTGCTGTGGAAATGATGAGGGATG
  GAGGGACAAGAGGAAGAATGAGAGGACACACGCCCTGGAGCCCTCACCACTGCCCTGGGGGTTGCCATCT
  GGAGGAGCCTGGGGATAAGGGTAACCCAGGGAGGCTGGGCACGAGGGAGCTGACTCCACGTTTTTCCCCC
  CGTTCCTCACCTTCGAGGGCCCTGCTAGGTCACCCCTATGCTGGCATGAAGAGCATGGGGCAATAAACCA
  GCACAGTCTCTGACCACTTGGAGCGTCTCATCCAGTGAGAGAGACAGCCGTTAAAAGCATAAACATCCAA
  ATAAAGATGCCTTTCCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004556.2
• Summary: 'The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]'
• Locus ID: 4794