FAK (PTK2) (NM_153831) Human 3' UTR Clone

CAT#: SC212185

3`UTR clone of PTK2 protein tyrosine kinase 2 (PTK2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PTK2
• Synonyms: FADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71
• ACCN: NM_153831
• Insert Size: 1070 bp
• Sequence Data:
  Insert Sequence

  >SC212185 3'UTR clone of NM_153831
  The sequence shown below is from the reference sequence of NM_153831. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AATGCTTGGGCAGACGAGACCACACTGAGCCTCCCCTAGGAGCACGTCTTGCTACCCTCTTTTGAAGATG
  TTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGCTCCAAC
  TTTTTTGAATGACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCAT
  TTTTTGTTTTGTTTTTTTCAATCATGATATTCAGAAAAATCCAGGATCCAAAATGTGGCGTTTTTCTAAG
  AATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACATTAAGATACGTTCTAA
  AGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGAGAGCAT
  GAAGCAAAGAATTCCAGGAAACCCAAGAGGCTGAGAATTCTTTTGTCTACCATAGAATTATTATCCAGAC
  TGGAATTTTTGTTTGTTAGAACACCCTTCAGTTGCAATATGCTAATCCCACTTTACAAAGAATATAAAAG
  CTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCTTTTACTTT
  CGTGTGGATATGTGAAGCATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTC
  ACAGAATAATGTGCCAGTTTTTTGTAGCAATGTTATTTCTCTTGGAAGCAGAAATGCTTTGTACCAGAGC
  ACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTTTTATATAATTTATAAAGA
  AAGATTAAAGCCATGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCC
  AGGAGAGAATGAAGCAAAACAGGAATTTGGTTTTCTTTTGATGTCCAGTTACACCATCCATTCTGTTAAT
  TTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCAGGAAGAATATAATGAAC
  TGTACAGTTACTTTGACCTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_153831.2
• Summary: 'This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]'
• Locus ID: 5747