XLF (NHEJ1) (NM_024782) Human 3' UTR Clone

CAT#: SC212243

3`UTR clone of nonhomologous end-joining factor 1 (NHEJ1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NHEJ1
• Synonyms: XLF
• ACCN: NM_024782
• Insert Size: 1074
• Sequence Data:
  Insert Sequence

  >SC212243 3'UTR clone of NM_024782
  The sequence shown below is from the reference sequence of NM_024782. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCTGTCAAAGGTCAAGAGGAAGAAGCCAAGGGGTCTCTTCAGTTAATCTGTTGTGGCCTCAGCTGCTGAG
  GATGGACTTGGAGAACAGCTTCCAAGCTTCACCTTGAAAGAAGCTTACATGGCAGCAATATTTCTAAAAT
  AGTGATACAGTCAGAGGCCTCCTGTAAGGGCGAGAGAACTGAAGTTGATGTTGACAGGCCCACAGGGAAT
  TGGCCTTCCCTGTTCAAGTGGAAGCCAGTCTCTGAGAATCCCGTGCTCTCCTCTCTTTTGGTGGAGGTTC
  TGTAGGTTCAGGTTTCTACCATGGACTTTAGGTATATAGGGCAAGTCAGCAAGAAAGCACCACACACTCA
  GGAAGCCTTGTCTACCTTTCCCTAGCGTCTCTAGCCAGCCAGCCCCAGATACTCCTCAGAGACCCACTTC
  TCTCTTTTGCATGGAATAAAAAGCACTCACAGTCCCTGCTTTTGGGATTACTTATGCTGTGGAACTCATA
  ACCCAATTCACTTCCTTCACTGGGTCCCACCCCATTGTTGTCTCTGGATGAAGTCTAGCCATCAGTCTGG
  TCCTTCAGATTCTTCGTAAACTTTTCTGCAGTTGCCAGAGCTAAGTCTCCATTTGGAAACTTTGATCTCA
  AGAACTCTCTTGATGGTGGGCACAAGACAGATAGGTGATTGCTGCTTTCCTTTGCCAAACTTGGATGCAT
  TTTTCTCTTTGTTTCCAAAGTATAGGCAACAGGTTAAACTACCACGTGGGGTAGTTTATCAATCCTCCCG
  CCTCATCCTTCTGCATAACTGGGACTAAAGGCACACACCACCACACCTAGCTAATTTTTGTATTTTTTTG
  TGTGTGGAGACAAGGCTTTGCCATGTTGCCCAGGCTGGTCTTGAACTCCTGGACTCTAGTGATCTGCCCA
  CCTCAACCTCCCAAAGTGCTGGGATTACAGGCATGAGCTACCACGCCTATCCAATTTCTGGTTTATTTAC
  TTTCGATGGTTAAATGCCTCCTTATTCAAATATCCTGTATATATACTGGCGTTTCTGTCCTGATGTCAAG
  TCTCGAGAGAAGATGATCAAACAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_024782.2
• Summary: Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]
• Locus ID: 79840