PPP2R5D (NM_006245) Human 3' UTR Clone

CAT#: SC212292

3`UTR clone of protein phosphatase 2 regulatory subunit B' delta isoform (PPP2R5D) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PPP2R5D
• Synonyms: B56D; B56delta; MRD35
• ACCN: NM_006245
• Insert Size: 1087 bp
• Sequence Data:
  Insert Sequence

  >SC212292 3'UTR clone of NM_006245
  The sequence shown below is from the reference sequence of NM_006245. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCGGAAGAGTTCCTAACTGCCAGCCAGGAGGCTCTCTGACCCCTCACGTTCCTACCACAGGGCCACAGCC
  CACACAGCCCTGGGACACTGCCCTGGCCCTCCATACTCTGCTCCCTACTGGCTGTCTTGGGGGAAGGCAG
  CGCCTCTCTAGCTACTCAAGGGAGGGGGATGTGGGCACTTGAAGCAGGGACACCCACAGAATGGTCCCTC
  TTCTCCCCAAAAGGTGTTCATGCCTCCCTGTGGCTAGTACAGGCTGAGCACTAAGATGCTTAGTGCTCAG
  ACAACCTGGGGATGCCTGTCCCCTACCTGCTCCTCACCCACAGCTACCTGAGGCTGCTCTGAGAAGTACA
  CACAGGAATACATACGCTCCTCTATTCTTCCCTTCATCCTCATTTGAACGCCAGGTATCTCCCCTCCTCT
  CTCTCCCCTGCAGAGGCATGTAGGGAACAGCAGGAGATTATTCTCACCAAAGTTATGTCAAGCCCCATTG
  GTCCCAGAGTAGCTGAAGGGAAGCCAACCCCCCTGCAGCACAAATAGGCCCCCCAGTCCCAGCCGTGTGC
  TGGCAGATAGGGTTGTATTATTTCTTCTTACCCCATGCCTGACCAAGGGAGGTCAAAAGGAGAAAAGTAT
  AGGCTGTGGACAATAACTGATGAATATAGGGCCCAGATGGACCAAGTGGGGCCGGGGAGGGATGAATAAA
  CACCCTACCCCGTGCCCTGTCTTTGGTGAGCAGCAGCCCTGGGGTCACAGACATGGAAGGGACCACCCTG
  GGGCTGACTGCTTTTCTGTGCTGTTGGTTCCCAAAACTAGAAAGAAGGAAGCAGGGAGCGGTGCCCCAAG
  CATGGCTCCTGCCAACACCTATTTATTTCCTTGTTTGTGCTATGCTGGGCAGGCCTTCTCTTGTCCCTTA
  TAGGTACCTTGGAGGGGCCAGGGGCTGAGGAAGGCCGGACCCAGGTTCCAGGGGCGCAGGCAGTGCGGCT
  TTTGGCTGTGTACATAGGGTGCTTTATTCTCCACAGAGTGATACATGCTAAGGTGGGTTGGGCTTGGACC
  GATGTCCCCATATGTACAGAACTGAATAAAGTGGGTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006245.2
• Summary: 'The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
• Locus ID: 5528