Caspase 8 (CASP8) (NM_001080124) Human 3' UTR Clone

CAT#: SC212577

3`UTR clone of caspase 8 apoptosis-related cysteine peptidase (CASP8) transcript variant F for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CASP8
• Synonyms: ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5
• ACCN: NM_001080124
• Insert Size: 1158 bp
• Sequence Data:
  Insert Sequence

  >SC212577 3'UTR clone of NM_001080124
  The sequence shown below is from the reference sequence of NM_001080124. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGCCTACTTTCACACTAAGAAAAAAACTTGTCTTCCCTTCTGATTGATGGTGCTATTTTGTTTGTTTTGT
  TTTGTTTTGTTTTTTTGAGACAGAATCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGC
  TCACCGCAAGCTCCGCCTCCCGGGTTCAGGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACA
  GGGGCCCGCCACCACACCTGGCTAATTTTTTAAAAATATTTTTAGTAGAGACAGGGTTTCACTGTGTTAG
  CCAGGGTGGTCTTGATCTCCTGACCTCGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGG
  CGTGAGCCACCGCGCCTGGCCGATGGTACTATTTAGATATAACACTATGTTTATTTACTAATTTTCTAGA
  TTTTCTACTTTATTAATTGTTTTGCACTTTTTTATAAGAGCTAAAGTTAAATAGGATATTAACAACAATA
  ACACTGTCTCCTTTCTCTTATGCTTAAGGCTTTGGGAATGTTTTTAGCTGGTGGCAATAAATACCAGACA
  CGTACAAAATCCAGCTATGAATATAGAGGGCTTATGATTCAGATTGTTATCTATCAACTATAAGCCCACT
  GTTAATATTCTATTAACTTTAATTCTCTTTCAAAGCTAAATTCCACACTACCACATTAAAAAAATTAGAA
  AGTAGCCACGTATGGTGGCTCATGTCTATAATCCCAGCACTTTGGGAGGTTGAGGTGGGAGGATTGCTTG
  AACCCAAGAGGTCAAGGCTGCAGTGAGCCATGTTCACACCGCTGCACTCAAGCTTGGGTGACAGAACAAG
  ACCCCGTCTCAAAAAAAATTTTTTTTTTAATAAAACAAAATTTGTTTGAAATCTTTTAAAAATTCAAATG
  ATTTTTACAAGTTTTAAATAAGCTCTCCCCAAACTTGCTTTATGCCTTCTTATTGCTTTTATGATATATA
  TATGCTTGGCTAACTATATTTGCTTTTTGCTAACAATGCTCTGGGGTCTTTTTATGCATTTGCATTTGCT
  CTTTCATCTCTGCTTGGATTATTTTAAATCATTAGGAATTAAGTTATCTTTAAAATTTAAGTATCTTTTT
  TCAAAAACATTTTTTAATAGAATAAAATATAATTTGAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001080124.1
• Summary: 'This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]'
• Locus ID: 841