PLAGL1 (NM_001080954) Human 3' UTR Clone

CAT#: SC212650

3`UTR clone of pleiomorphic adenoma gene-like 1 (PLAGL1) transcript variant 6 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PLAGL1
• Synonyms: LOT1; ZAC; ZAC1
• ACCN: NM_001080954
• Insert Size: 1150 bp
• Sequence Data:
  Insert Sequence

  >SC212650 3'UTR clone of NM_001080954
  The sequence shown below is from the reference sequence of NM_001080954. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGCCTCATTTCCATCATGCATTCAGATAATTGATTTTTAAAGTGTATTTTTCGTATTCTGGAAGATGTT
  TTAAGAAGCATTTTAAATGTCAGTTACAATATGAGAAAGATTTGGAAAACGAGACTGGGACTATGGCTTA
  TTCAGTGATGACTGGCTTGAGATGATAAGAGAATTCTCGAACTGCATGTATTGTGCCAATCTGTCCTGAG
  TGTTCATGCTTTGTACCAAATTTAATGAACGCGTGTTCTGTAATCAAACTGCAAATATTGTCATAACCAA
  CATCCAAAATGACGGCTGCTATATATAAGTGTTTGTCATATGGAATTTAATCGTAAGCCATGATCATAAT
  GTTAACTAAATAACTTTATGTGGCACTGCCTAGTAAGGGAACTATGGAAAGGTTTGGATTTCTCCAAATC
  TGGGAGAATTTTCAAAATAAGAAAATAACCTTTATATGATATACTATGACTAGGCTGTGTATTTCTTTTC
  AGGGATTTTTCTACCTTCAGGGTTGGATGTAGTTTAGTTACTATTACCATAGCCAACCTGTAGTTTTACA
  TATACATTTTCTTGTGGAGCAATAGAGTTCTCCATTTTACAGAAGCATTTTAAATGTAGTTTGAATATTT
  TCCACAAGATGCTGCAATGTGAGTTATCACTTCATTTATCTTAAAGAAAGACTAAACTGGTTGTCAGTTA
  CATCTGACAGAAAAAAAAAAAAAATCACTGTGTAACCAGGTTAAGTGGTAAAATAATCCAGGCGTCAGTC
  AAAGGCATTTTGCTGACTTTAATATTGATTATATTTTTAACAGGAATTTAAGAAAATATTACTGGAATTA
  AAAATATATATATATTAAACAAGAATTTTCTTTGCTCTGTCTAGCTTAAACTACTACTCAAGCTGCTTAA
  GTTCTTAAGTATTGTTTGTAATCACCAATAAATAAGTGCATTTGTAATTCATCAGTCATTATTAGCTTTT
  ATTAAAAGAAGATTACGTTTTACAATGTAACTATAATCTCTTGAATTTGGTATCTTATTAATGAGTTTTA
  AAGATGTAAAACCTAACCTTTTTTAAAGCTCCATTGTCTTATGTTTTTAGAGGCTTTTCCGTAAACATAT
  ATCTTACATATAATAAACTTTTCAAATCTT
  
  CGGACCGTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001080954.1
• Summary: 'This gene encodes a C2H2 zinc finger protein that functions as a suppressor of cell growth. This gene is often deleted or methylated and silenced in cancer cells. In addition, overexpression of this gene during fetal development is thought to be the causal factor for transient neonatal diabetes mellitus (TNDM). Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding two different protein isoforms. The P1 downstream promoter of this gene is imprinted, with preferential expression from the paternal allele in many tissues. [provided by RefSeq, Nov 2015]'
• Locus ID: 5325