SLUG (SNAI2) (NM_003068) Human 3' UTR Clone

CAT#: SC212661

3`UTR clone of snail homolog 2 (Drosophila) (SNAI2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SNAI2
• Synonyms: SLUG; SLUGH; SLUGH1; SNAIL2; WS2D
• ACCN: NM_003068
• Insert Size: 1130 bp
• Sequence Data:
  Insert Sequence

  >SC212661 3'UTR clone of NM_003068
  The sequence shown below is from the reference sequence of NM_003068. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGAATCTGGCTGCTGTGTAGCACACTGAGTGACGCAATCAATGTTTACTCGAACAGAATGCATTTCTTC
  ACTCCGAAGCCAAATGACAAATAAAGTCCAAAGGCATTTTCTCCTGTGCTGACCAACCAAATAATATGTA
  TAGACACACACACATATGCACACACACACACACACACCCACAGAGAGAGAGCTGCAAGAGCATGGAATTC
  ATGTGTTTAAAGATAATCCTTTCCATGTGAAGTTTAAAATTACTATATATTTGCTGATGGCTAGATTGAG
  AGAATAAAAGACAGTAACCTTTCTCTTCAAAGATAAAATGAAAAGCACATTGCATCTTTTCTTCCTAAAA
  AAATGCAAAGATTTACATTGCTGCCAAATCATTTCAACTGAAAAGAACAGTATTGCTTTGTAATAGAGTC
  TGTAATAGGATTTCCCATAGGAAGAGATCTGCCAGACGCGAACTCAGGTGCCTTAAAAAGTATTCCAAGT
  TTACTCCATTACATGTCGGTTGTCTGGTTGCCATTGTTGAACTAAAGCCTTTTTTTGATTACCTGTAGTG
  CTTTAAAGTATATTTTTAAAAGGGAGGAAAAAAATAACAAGAACAAAACACAGGAGAATGTATTAAAAGT
  ATTTTTGTTTTGTTTTGTTTTTGCCAATTAACAGTATGTGCCTTGGGGGAGGAGGGAAAGATTAGCTTTG
  AACATTCCTGGCGCATGCTCCATTGTCTTACTATTTTAAAACATTTTAATAATTTTTGAAAATTAATTAA
  AGATGGGAATAAGTGCAAAAGAGGATTCTTACAAATTCATTAATGTACTTAAACTATTTCAAATGCATAC
  CACAAATGCAATAATACAATACCCCTTCCAAGTGCCTTTTTAAATTGTATAGTTGATGAGTCAATGTAAA
  TTTGTGTTTATTTTTATATGATTGAATGAGTTCTGTATGAAACTGAGATGTTGTCTATAGCTATGTCTAT
  AAACAACCTGAAGACTTGTGAAATCAATGTTTCTTTTTTAAAAAACAATTTTCAAGTTTTTTTTACAATA
  AACAGTTTTGATTTAAAATCTCGTTTGTATACTATTTTCAGAGACTTTACTTGCTTCATGATTAGTACCA
  AACCACTGTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003068.3
• Summary: 'This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008]'
• Locus ID: 6591