DPYD (NM_001160301) Human 3' UTR Clone

CAT#: SC212663

3`UTR clone of dihydropyrimidine dehydrogenase (DPYD) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DPYD
• Synonyms: DHP; DHPDHASE; DPD
• ACCN: NM_001160301
• Insert Size: 1172 bp
• Sequence Data:
  Insert Sequence

  >SC212663 3'UTR clone of NM_001160301
  The sequence shown below is from the reference sequence of NM_001160301. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGAGACTTTGATCCTGGCTTTCTCTTTAATGAATCATTTGTAAAGACTTTGATTCTTCAGACACTCTTG
  GGCTCTCTGACGTCTCCTATTTGGAGTCAACAAGCAGGTTAATTCCCATGTATCATTTAAATGATCAGCT
  TATATGAAGGGTATAGTAAGATTTTATAATCTTTGGAGACTAGTTGGCTAGAAGGTCAAATTAAGTGCCC
  GTCAACCTAGATTTTTAGTTAATGACTTACTGATCTGGTTTTGTCAGCATAAACACAATGTGTTCTTTCA
  TTTTGGGACCCTTCCCATAATATTCCTCAAGCTTAAGTCTCACTTTGCTCTTAGTAATAAATCAGGGTTA
  TTCCCTTCCTGCTTTCTGACAGGTTAATTCTAAAGAAGAGCTCCTGGGAATAAGAAAATAGAAACAATTC
  GAGGTTGTGGATTGTTGCAATAAATTCTGGGATTTAATTTCTTTTCTTACCAAATTCAAAATTGCATTAG
  GCTTACCTGACCACTACGAAGTATGATCTTTACTACCTGTGAAAATCCTTAGTTGATGGAGAAGATTCAC
  TTGAATTCCTCCTCACTTTTCTTTGGGGACCCATTGCCTCTTCATTAAACCAGGGCAAGCTTTCACAGAT
  GCAGTAAGTGAAAGGTCACATAGTATGTATATCAACTAACACAGCATTTTAAAACAAAATGAATTAAAAT
  GATATCATTAGTAATTGATCTGGTCATGAGAGGTGTGTGTGTTTGTGTGTGTGTGTGTGTGTGTGTGAGA
  GAGAGAGAGAGAAAGAGAGAGAGAGACAGGGAAATACATTATTCCTCTGGAAACTCTCACATGGTTTTAA
  TTCTTTTCTCTGGGAACACTGTCCCTCTGGGAACTCTTACTTGGTTCTACCACTCATTCCCAGAGCAGAG
  TCTATTTAAGACAGAGACATGTGCCTTCAGAACAGAATTTATTAGAAACTTACTAAAATTTGAGAGTGGT
  CACACTTAGGTCTTGCCATAAGTATCACCTAATGAAGAGTTATGCATAGATTAGGACTGCAGTAGATGCA
  TTTTAAGTGAATCAAGGAATTTAGAATAGGAATGTATCTGCTTATTTCATTCCAATTATAGTTTGTATAA
  TTGTAATTTCAATATCAAATTATAAATTAAAAATGTCCTCGTGGGAACTACT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001160301.1
• Summary: 'The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]'
• Locus ID: 1806