NUP98 (NM_016320) Human 3' UTR Clone

CAT#: SC212817

3`UTR clone of nucleoporin 98kDa (NUP98) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NUP98
• Synonyms: ADIR2; NUP96; NUP196
• ACCN: NM_016320
• Insert Size: 1188 bp
• Sequence Data:
  Insert Sequence

  >SC212817 3'UTR clone of NM_016320
  The sequence shown below is from the reference sequence of NM_016320. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCAGTCCTATCTGCGAGAACTGGCTGTTGGGAGCCTGTGAGCCCCAGGCACTTTGCATCACAGTCACATG
  CCCATTCACACCACACAGAGGTTCCCTGCCTTGTTTGGATTGGCACTGTTTGCCATTCTCTGGGTTGGCT
  GTGGCATCTACCCTCCCTCCCTGCTGCCAGAAGCAGCATCCTCCACTTGTTCAGGGCTTTTCTTAATACT
  GAACGTAGCATAAGGGCTTCTGGAACCCAGAAGAGGAGACAGTTTACCATCCTCAAGATCATTCAGTGTT
  TTTCCTTTAAAAAAATGGTCAATAAAGCTCCTTTGGCAGAATCCCCCAAAGAACCAGGGTATTCTTTTTC
  CATCCCTAGCCATTCTGGATCTTGTGACCCTCCATGCCAACCAGCTTCCCTACTCCTACCCTGGCCCTTT
  TATACTAGGACTCCTTAGGAGGAGTGAGACAGGTGATAATGGATCCTTAACAGATGAACTATCCACAGAA
  GGAAGAGGGATCCGTCTCTTAAGTAATTGGTTAGTTAACACTGAATTTTGGAGGCAAAGGAGGGTTGGCC
  TGAGTTAGGAAACAAAATGGGATCTTTCTGACACACTTAGGGCAGAAGTGAATGCCTGTCACGGAGGGAT
  TGATCTTCAGGGCTGTTTTTGTTCCTGCCTTTAGAGTTCCATGAACACCATACCTTTGCTACTACTATGT
  GCAGGAACCCTTGGTCACATGTGACATGTCTGTGGGAAGCTCCCAGAGTTTGGTTTGGTCCCTGGTTTTC
  AGTCTTGCTGAGACTCTGTCTGGATTTGCCTGCAGAGTTTGGATAAAAAATGGCAGGTTGGGTAACCCTC
  CCTGTTCATCCCATGTTAGCTCCAAAGCATTTCCCACCCTCCATCTACCCCTTCCAGAAGCAAAAACAAA
  CCATGACTGAGGCAGGCATGGAGTTGGGCGTTAGGGGCAGGCAGAGGGCCTTTGCTACACTGCTGACAGC
  TATAGGGAGCCCCAGGTAATGGCATGAGATAGCTGGTGTTAGGGCTATCTCAGGCAATATGGCCACACCT
  GGGTCTTTATGCATGAAGATAATGTAAAGGTTTTTATTAAAAAATATATATATGTATAAATAAATGATCT
  AGATATTTTCCTCTTTTTCTGAAGCTACTTTCTTAAAAAATAAATAAAATGTTTATAGCATTCCTGGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_016320.3
• Summary: 'Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. [provided by RefSeq, Feb 2016]'
• Locus ID: 4928