TFII I (GTF2I) (NM_001518) Human 3' UTR Clone

CAT#: SC212857

3`UTR clone of general transcription factor IIi (GTF2I) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GTF2I
• Synonyms: BAP135; BTKAP1; DIWS; GTFII-I; IB291; SPIN; TFII-I; WBS; WBSCR6
• ACCN: NM_001518
• Insert Size: 1200 bp
• Sequence Data:
  Insert Sequence

  >SC212857 3'UTR clone of NM_001518
  The sequence shown below is from the reference sequence of NM_001518. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGGAAGCTCTCAGATCAAGCAAGAACCAGACCCCACGTGGTAGACCTCTTCCCTCCTAGGCTTAAAGTAT
  CAGTGGTTGAGAAGAGCTTTTCGGACCTGTTACTACCCCAAGCTGTGTAATATACTTGTATAACAGAAAT
  ACCTTCTATACAAACCTTTTTTTCTACTTTTAGATAGAAATGTCTACTTTTTCAGCAGTTCTGTGAATTA
  AAGAGCAGAGTGACTGTGGGTCTGGAATGGCTGGTGTACTTGGGAATGTACTATCAGGATTTTACAGCAA
  TGCTGGGAAATGACAGGGAAAATGACAGGAATGAATCTCACCAGATTTTTTATGTACTCAGCAGAGCCTT
  GAGTTACGGTGTTTATTTTCCAATCAAGTGAAGATATCTCCTACTTCTCCTACTGGAACATCTCAGCTTC
  TGCAGTGAAGAAAAATTCCTGTGATAGTTCAGTTCTTTAGTTTTTCTATTTGAAAAAAAAAAATCATTTA
  AATGATCCTTTGTTCACGGCTCTCCTTAATGACTGAGTGAACAGTTCCTATCTGTATATTTGACTAAACC
  TTTTCCTAAGCTATCTCTCATGGTTCCTATGTTTTTTTATCATAATTAAAAGCAAAACCATCTGGATCAC
  CTAACAGTCAGAGGTCAGTATCTCAGCGTGTGAATTATAGAGGAAATACAGAGAGAACCTCTTCCACTTT
  TACTTTTCGTCCAAATAAAATGCATGGTGTACCAGAAGTTGAAGATCGGGTTGAGGATTGGGGCTAGCTC
  GATGACACTAAGGCCCCAACATCGCGGGACCTGCTGTGGCGCGGATTCTTAGGAACGCTGTTCTAGCCGG
  CCCCCTCTCCAGGGGTCGCCGTGGCCGGCATTATTTCCTAGTTCTTCTTGTAACCCTGAGGTGCCAGCGC
  GGGGAGTGAGGAGGGGTCAGGGGGCTAAGGATGCAACCTCTGACGTTCTGCGCCTTCCTAGGAGAGTCTT
  ACATGTGTTGAGATTTCACAAGCAATGCGAGTTGTAAAATACCAGCTCTACAAGAAGCTAGGCTCTGTGA
  CGGCATAGTTTTCAGTAGCTTTATCACAATATTCACAATGGAGAATTATATGACATGGTAGCAGAAATAG
  GCCCTTTTATGTGTTGCTTCTATTTTACCTCAAATTGTAGATATAGGGTAATCAATAAAATCCATCCATG
  CCTTTCACAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001518.3
• Summary: 'This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]'
• Locus ID: 2969