PKC eta (PRKCH) (NM_006255) Human 3' UTR Clone

CAT#: SC212876

3`UTR clone of protein kinase C eta (PRKCH) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PRKCH
• Synonyms: nPKC-eta; PKC-L; PKCL; PRKCL
• ACCN: NM_006255
• Insert Size: 1151 bp
• Sequence Data:
  Insert Sequence

  >SC212876 3'UTR clone of NM_006255
  The sequence shown below is from the reference sequence of NM_006255. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCTATGTGTCTCCAGAATTGCAACCATAGCCTTATGGGGAGTGAGAGAGAGGGCACGAGAACCCAAAGGG
  AATAGAGATTCTCCAGGAATTTCCTCTATGGGACCTTCCCAGCATCAGCCTTAGAACAAGAACCTTACCT
  TCAAGGAGCAAGTGAAGAACTCTGTGAAGGATGGAACTTTCAGATATCAACTATTTAGAGTCCAGAGGGA
  GCCATGGCACTAGAAATAGTTGATAATGAAATGAGATTTTATGAAGTATACCGCTCCACCTATGAGCGTC
  TGTCTCTGTGGGCTTGGGATGTTAACAGGAGCCAAAAGGAGGGAAAGTGTGAAGAATAAAGTAGATCTGA
  GAAATTCTGAGCCAATCAGGCTTCTTAATTCAAGAGACAAACCAAGACGTTCTGTCAACTGTGCTGTGCT
  CTTCTTTAAGCCAATGAACCCCAATTCCTGGCAGTCTACAAGAAGTCTCTTAATGCTAATGAAGAATTTA
  AAGGTCTTTTTAAGGAAATGAAGGGCTTTCCAAATAGAATGATTTACTCTGAAGAAACAAACAATGGTAT
  CTCTGAAACTCACAACCTAAAGCCCAATCTTGAAAATATGTTGTGCACCAAGACGACTGCTTCAGCTTCT
  TCTCTTATCCTTACTTTCTTTAATAGATATTTATTAAACTGTCCAGTGAAAAGGTGCCACAATGCCCAGT
  ATTGTAAACAACAGGTTTGCATTCATGAAGCTTTCATTCATTCTGGAGTCTACTAATTTACCTGAATGGT
  GTTTGCATTCTGTGAAATGCCTCTCCACGTTGCATATGTCACACTTTTGTCTGCACATAACTCTTTTTTC
  ACAAGAAGGGTCACTGCCACAACAGCACAGTCAGCGGGTGAATTACAGGTGCCTGCTGCCTGCCTACCTG
  GGTAATCTGATCTTGTCTGTATCGCCGTGTGCTCATCACTGAAGAATTGCAGGCCACTCATGTCAGTGAC
  CAGATTTGTGGCTTATAAACATTAGCAGTTTATTTATGTTTTAAGATGCAAAGATGTGTGTTTGATATTC
  ACTTTAATAATTAGAAATGGATCTTGTAAACAGGGCATATATCAAAGATGACCTTATAATATGTACCCGA
  ATATACAGTTCAAGAATTTTGTCTGACTGGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006255.3
• Summary: 'Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]'
• Locus ID: 5583