CD133 (PROM1) (NM_001145851) Human 3' UTR Clone

CAT#: SC212895

3`UTR clone of prominin 1 (PROM1) transcript variant 5 for miRNA target validation


Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol PROM1
Synonyms AC133; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4
ACCN NM_001145851
Insert Size 1189
Sequence Data
>SC212895 3'UTR clone of NM_001145851
The sequence shown below is from the reference sequence of NM_001145851. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

CGATGATGTTGAAACTATACCCATGAAAAACCCATCACAACATTGATAGCTGATGTTGAAACTGCTTGAG
CATCAGGATACTCAAAGTGGAAAGGATCACAGATTTTTGGTAGTTTCTGGGTCTACAAGGACTTTCCAAA
TCCAGGAGCAACGCCAGTGGCAACGTAGTGACTCAGGCGGGCACCAAGGCAACGGCACCATTGGTCTCTG
GGTAGTGCTTTAAGAATGAACACAATCACGTTATAGTCCATGGTCCATCACTATTCAAGGATGACTCCCT
CCCTTCCTGTCTATTTTTGTTTTTTACTTTTTTACACTGAGTTTCTATTTAGACACTACAACATATGGGG
TGTTTGTTCCCATTGGATGCATTTCTATCAAAACTCTATCAAATGTGATGGCTAGATTCTAACATATTGC
CATGTGTGGAGTGTGCTGAACACACACCAGTTTACAGGAAAGATGCATTTTGTGTACAGTAAACGGTGTA
TATACCTTTTGTTACCACAGAGTTTTTTAAACAAATGAGTATTATAGGACTTTCTTCTAAATGAGCTAAA
TAAGTCACCATTGACTTCTTGGTGCTGTTGAAAATAATCCATTTTCACTAAAAGTGTGTGAAACCTACAG
CATATTCTTCACGCAGAGATTTTCATCTATTATACTTTATCAAAGATTGGCCATGTTCCACTTGGAAATG
GCATGCAAAAGCAATCATAGAGAAACCTGCGTAACTCCATCTGACAAATTCAAAAGAGAGAGAGAGATCT
TGAGAGAGAAATGCTGTTCGTTCAAAAGTGGAGTTGTTTTAACAGATGCCAATTACGGTGTACAGTTTAA
CAGAGTTTTCTGTTGCATTAGGATAAACATTAATTGGAGTGCAGCTAACATGAGTATCATCAGACTAGTA
TCAAGTGTTCTAAAATGAAATATGAGAAGATCCTGTCACAATTCTTAGATCTGGTGTCCAGCATGGATGA
AACCTTTGAGTTTGGTCCCTAAATTTGCATGAAAGCACAAGGTAAATATTCATTTGCTTCAGGAGTTTCA
TGTTGGATCTGTCATTATCAAAAGTGATCAGCAATGAAGAACTGGTCGGACAAAATTTAACGTTGATGTA
ATGAAATTCCAGATGTAGGCATTCCCCCCAGGTCTTTTCATGTGCAGATTGCAGTTCTGATTCATTTGA

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_001145851.1
Summary This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Locus ID 8842

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