CISH (NM_013324) Human 3' UTR Clone

CAT#: SC212904

3`UTR clone of cytokine inducible SH2-containing protein (CISH) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CISH
• Synonyms: BACTS2; CIS; CIS-1; G18; SOCS
• ACCN: NM_013324
• Insert Size: 1168 bp
• Sequence Data:
  Insert Sequence

  >SC212904 3'UTR clone of NM_013324
  The sequence shown below is from the reference sequence of NM_013324. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGACTACCTCCGACAGTACCCCTTCCAGCTCTGACTGTACGGGGCAATCTGCCCACCCTCACCCAGTCGC
  ACCCTGGAGGGGACATCAGCCCCAGCTGGACTTGGGCCCCCACTGTCCCTCCTCCAGGCATCCTGGTGCC
  TGCATACCTCTGGCAGCTGGCCCAGGAAGAGCCAGCAAGAGCAAGGCATGGGAGAGGGGAGGTGTCACAC
  AACTTGGAGGTAAATGCCCCCAGGCCGCATGTGGCTTCATTATACTGAGCCATGTGTCAGAGGATGGGGA
  GACAGGCAGGACCTTGTCTCACCTGTGGGCTGGGCCCAGACCTCCACTCGCTTGCCTGCCCTGGCCACCT
  GAACTGTATGGGCACTCTCAGCCCTGGTTTTTCAATCCCCAGGGTCGGGTAGGACCCCTACTGGCAGCCA
  GCCTCTGTTTCTGGGAGGATGACATGCAGAGGAACTGAGATCGACAGTGACTAGTGACCCCTTGTTGAGG
  GGTAAGCCAGGCTAGGGGACTGCACAATTATACACTATTTATTTATTTATTCTCCTTGGGGTTGGTGTCA
  GGGGCGAGCCAACCCCACCTCTATGCCCTGAGCCCTGGTAGTCCAGAGACCCCAACTCTGCCCTGGCTTC
  TCTGGTTCTTCCCTGTGGAAAGCCCATCCTGAGACATCTTGCTGGAACCAAGGCAATCCTGGATGTCCTG
  GTACTGACCCACCCGTCTGTGAATGTGTCCACTCTCTTCTGCCCCCAGCCATATTTGGGGAGGATGGACA
  ACTACAATAGGTAAGAAAATGCAGCCGGAGCCTCAGTCCCCAGCAGAGCCTGTGTCTCACCCCCTCACAG
  GACAGAGCTGTATCTGCATAGAGCTGGTCTCACTGTGGCGCAGGCCCCGGGGGGAGTGCCTGTGCTGTCA
  GGAAGAGGGGGTGCTGGTTTGAGGGCCGCCACTGCAGTTCTGCTAGGTCTGCTTCCTGCCCAGGAAGGTG
  CCTGCACATGAGAGGAGAGAAATACACGTCTGATAAGACTTCATGAAATAATAATTATAGCAAAGAACAG
  TTTGGTGGTCTTTTCTCTTCCACTGATTTTTCTGTAATGACATTATACCTTTATTACCTCTTTATTTTAT
  TACCTCTATAATAAAATGATACCTTTCATGTACAGAGCCCTCAATGGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_013324.5
• Summary: 'The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]'
• Locus ID: 1154