GIRK1 (KCNJ3) (NM_002239) Human 3' UTR Clone

CAT#: SC213044

3`UTR clone of potassium inwardly-rectifying channel subfamily J member 3 (KCNJ3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: KCNJ3
• Synonyms: GIRK1; KGA; KIR3.1
• ACCN: NM_002239
• Insert Size: 1191 bp
• Sequence Data:
  Insert Sequence

  >SC213044 3'UTR clone of NM_002239
  The sequence shown below is from the reference sequence of NM_002239. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAACTCTGATCGCTTCACATAACAAAGCACTCCCTTAGGCATTATTTAATGTTTGATTTAGTAATAGTCC
  AATATTTGGCGATGAGGTAATTCTCCCTAAGGAATCTGAAAGTATATTTTCCTCCCAGTTCTACAAGCAT
  ATTTGAGAACCCTTCCTTTCCCAAGTATTGCGAATGTGCAGAAAGCAACAGTTACGGAGGGAGGACATCA
  TAAGGAAGTTATTAACGGGCATGTATTATCACATCAAGCATGCAATAATGTGCAAATTTTGCATTTAGTT
  TTATGGCATGATTTATATATGGCATATTTATATTGTATATTCTGGAAAAAAAATATATATATATATTTAA
  AGGGGAGATACTCTCCCTGACATTTCTAACATATGTATTAAGCCAAACATGAGTGAATAGCTTTCAGGGC
  GATAAAACTAAATATATGTCTGTGTGTGTGTGTGTATGTATACACACATATACATATATATATACACATA
  CATACACATACATACATACATACATATATATCTGATAAAATTGTGATGTTTTGTTCAAAGTTGTAGTTCT
  TGTGCATGTTTACTTTATTAGAGTAGGAAGGCTACTGGCATTAATTATTAATACCAAATATTTTAGCCTT
  AAATTTTTGTCATTTTAAAATCTGATTTAATGTTTTCTGCTGTTTAAGGTCTTGGGAGGCTTTCAATTGT
  ATTTTATATGAGAGAATCACACAAGTTTGTGCTATCTATGGCCCTGCAAAAATATAACCATTACATGTTT
  AAATTGTAAATTTTAGAGCATACCAGTACTCAGTATAGCATTGAACATTTCTTATGATTTTTAAAAGTTG
  CTAGTACTGGGGAGAAATAATTGTTGATTAATTTGAGAATTATTCCTTTCCTAGACTAATTAAAATCTGG
  AAATCTGTTTTGTATATGATCTAATACAAAGATGAGCTCTGAACAAACACTGAATCATGTTAATAGACAG
  TAGCCAAGTTATATTGAATATATCAGAATCTGTGTGAAGTTACACAATTAATTGTCCCTGTTTCAAACTG
  AGTAAATTGGAAACATTTTCTTTCTTTTTCTGGAAATTTTGTCCATTTTAAAAACCAATCATTTTAAGAA
  GACATGACAATGCAATGAAACAGATGATAAATATTTATGCTTAAAATATGTATGTCTAATTGAGTCTCTT
  T
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002239.2
• Summary: 'Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex that also couples to neurotransmitter receptors in the brain and whereby channel activation can inhibit action potential firing by hyperpolarizing the plasma membrane. These multimeric G-protein-gated inwardly-rectifying potassium (GIRK) channels may play a role in the pathophysiology of epilepsy, addiction, Down's syndrome, ataxia, and Parkinson's disease. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, May 2012]'
• Locus ID: 3760