LEF1 (NM_001130713) Human 3' UTR Clone

CAT#: SC213362

3`UTR clone of lymphoid enhancer-binding factor 1 (LEF1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: LEF1
• Synonyms: LEF-1; TCF1ALPHA; TCF7L3; TCF10
• ACCN: NM_001130713
• Insert Size: 1225
• Sequence Data:
  Insert Sequence

  >SC213362 3'UTR clone of NM_001130713
  The sequence shown below is from the reference sequence of NM_001130713. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGTCCAAGAATGACAGCTGCCTACATCTGAAACATGGTGGAAAACGAAGCTCATTCCCAACGTGCAAAGC
  CAAGGCAGCGACCCCAGGACCTCTTCTGGAGATGGAAGCTTGTTGAAAACCCAGACTGTCTCCACGGCCT
  GCCCAGTCGACCCCAAAGGAACACTGACATCAATTTTACCCTGAGGTCACTGCTAGAGACGCTGATCCAT
  AAAGACAATCACTGCCAACCCCTCTTTCGTCTACTGCAAGAGCCAAGTTCCAAAATAAAGCATAAAAAGG
  TTTTTTAAAAGGAAATGTAAAAGCACATGAGAATGCTAGCAGGCTGTGGGGCAGCTGAGCAGCTTTTCTC
  CCCTCATATCTGCGTGCACTTCCCAGAGCATCTTGCATCCAAACCTGTAACCTTTCGGCAAGGACGGTAA
  CTTGGCTGCATTTGCCTGTCATGCGCAACTGGAGCCAGCAACCAGCACATCCATCAGCACCCCAGTGGAG
  GAGTTCATGGAAGAGTTCCCTCTTTGTTTCTGCTTCATTTTTCTTTCTTTTCTTTTCTCCTAAAGCTTTT
  ATTTAACAGTGCAAAAGGATCGTTTTTTTTTGCTTTTTTAAACTTGAATTTTTTTAATTTACACTTTTTA
  GTTTTAATTTTCTTGTATATTTTGCTAGCTATGAGCTTTTAAATAAAATTGAAAGTTCTGGAAAAGTTTG
  AAATAATGACATAAAAAGAAGCCTTCTTTTTCTGAGACAGCTTGTCTGGTAAGTGGCTTCTCTGTGAATT
  GCCTGTAACACATAGTGGCTTCTCCGCCCTTGTAAGGTGTTCAGTAGAGCTAAATAAATGTAATAGCCAA
  ACCCACTCTGTTGGTAGCAATTGGCAGCCCTATTTCAGTTTATTTTTTCTTCTGTTTTCTTCTTTTCTTT
  TTTTAAACAGTAAACCTTAACAGATGCGTTCAGCAGACTGGTTTGCAGTGAATTTTCATTTCTTTCCTTA
  TCACCCCCTTGTTGTAAAAAGCCCAGCACTTGAATTGTTATTACTTTAAATGTTCTGTATTTGTATCTGT
  TTTTATTAGCCAATTAGTGGGATTTTATGCCAGTTGTTAAAATGAGCATTGATGTACCCATTTTTTAAAA
  AAGCAAGGCACAGCCTTTGCCCAAAACTGTCATCCTAACGTTTGTCATTCCAGTTTGAGTTAATGTGCTG
  AGCATTTTTTTAAAAGAAGCTTTGTAATAAAACAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001130713.2
• Summary: This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
• Locus ID: 51176