SMURF 2 (SMURF2) (NM_022739) Human 3' UTR Clone

CAT#: SC213364

3`UTR clone of SMAD specific E3 ubiquitin protein ligase 2 (SMURF2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SMURF2
• Synonyms: DKFZp686F0270; MGC138150
• ACCN: NM_022739
• Insert Size: 1270
• Sequence Data:
  Insert Sequence

  >SC213364 3'UTR clone of NM_022739
  The sequence shown below is from the reference sequence of NM_022739. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCTGCTAACAGCCATTGAAGAAACATGTGGATTTGCTGTGGAATGACAAGCTTCAAGGATTTACCCAGGA
  CTCTATTTATACAACCCTGACTGACAGCCTCCTTTCAGCAGAGTTTCAAAGAATATGCTGAAATACAGGA
  AAACACTCCCCCCCCCCCCCTTTTTTTTTTTTTTTTTACATTTTAGGACACTGTGAGGGGAAAGGACAAT
  TTTGAAATTCCTTTTCAAGGAAAAAAAAGGTCTTTATGCTTTGCCATGAGGCCACATTCAGCTGCTATTT
  AAACTTAATATCTTGAACCTAAAGAATGCTGACTTTTCCTACATTTCCAGAGTTAGGCAGTATTCTACAC
  TTAAAGACTACTACTATTTTTATAAAAGGTAATCTATTCAAATTTCTTCACAGATTTCAAGTCTCTCAAA
  CATCAAGACAACTTCAGCAGTCGGTACAAGTCACATTTCATTTTGATTGAATACATGATCTTGAACAGCT
  CCTGTACTTGCTCTTTGTAAAAAAAAATAAAATTATTTTGAATTATTCTACCTTTGTAAACAATTGGCTA
  AAAGAATCATCTTTAAGAAATTAAGCCATTTACATGTTTGTGTTTTTCTATAGCAGAGCATTATATTTTG
  CATTATATGTTTCAACCTAGTCTAAGTGGGTCTTTTTTACATTTTTCAAGAACGGATTTCCTGGAATACA
  GCGATATAATTTTGGTTGTCAAATTCCTAATGCAACCATTTAGTCTAAACTTAGTCATTTATTTGTGACA
  ATAAGATGTGTTCAGGGGCTCCCTGTTTTTAAGAGACTCTTTTAAAAAAAAAAAAACCTAATGTTTTTAT
  CTTGAGTCAATATGATTAGGTATTTTGGATTTACTTTTAATCTTAAAATACTGCATTTTTATAGCTTCTC
  AGAGCATGTGGATGGGATGGGATTTTCGTTATTTTGCTGGGTCAGCTTATCTTTAATATATGGACTATTC
  CTATAAACCAAAGTCTCTGACAAGTGCACCTAATTTATATTGTATTTTAACTACAGTGTAAGTTTCCATT
  AACAAAACCATCCTAAAGCGTTAACTGCTCATAATTTTAATCAGCTACAGTTATGAAAAAGGAAGAATTT
  TGCTCTAAAGATTATTAATAAGCTTAGAAAATCCTGCTTTTACCTAACAGAATGAAGTGGGGTTGAGGGG
  CGGTAGTATGAACAGTGATTCTAACTCTATGGCGTAAATTACTTTGAAGAGTTCATTTTGGAAGTAAGGG
  AATCCCGCTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_022739.3
• Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. [UniProtKB/Swiss-Prot Function]
• Locus ID: 64750