COMT (NM_007310) Human 3' UTR Clone

CAT#: SC213428

3`UTR clone of catechol-O-methyltransferase (COMT) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: COMT
• Synonyms: HEL-S-98n
• ACCN: NM_007310
• Insert Size: 1266 bp
• Sequence Data:
  Insert Sequence

  >SC213428 3'UTR clone of NM_007310
  The sequence shown below is from the reference sequence of NM_007310. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGAAGGCCATCTACAAGGGCCCAGGCAGCGAAGCAGGGCCCTGACTGCCCCCCCGGCCCCCCTCTCGGGC
  TCTCTCACCCAGCCTGGTACTGAAGGTGCCAGACGTGCTCCTGCTGACCTTCTGCGGCTCCGGGCTGTGT
  CCTAAATGCAAAGCACACCTCGGCCGAGGCCTGCGCCCTGACATGCTAACCTCTCTGAACTGCAACACTG
  GATTGTTCTTTTTTAAGACTCAATCATGACTTCTTTACTAACACTGGCTAGCTATATTATCTTATATACT
  AATATCATGTTTTAAAAATATAAAATAGAAATTAAGAATCTAAATATTTAGATATAACTCGACTTAGTAC
  ATCCTTCTCAACTGCCATTCCCCTGCTGCCCTTGACTTGGGCACCAAACATTCAAAGCTCCCCTTGACGG
  ACGCTAACGCTAAGGGCGGGGCCCCTAGCTGGCTGGGTTCTGGGTGGCACGCCTGGCCCACTGGCCTCCC
  AGCCACAGTGGTGCAGAGGTCAGCCCTCCTGCAGCTAGGCCAGGGGCACCTGTTAGCCCCATGGGGACGA
  CTGCCGGCCTGGGAAACGAAGAGGAGTCAGCCAGCATTCACACCTTTCTGACCAAGCAGGCGCTGGGGAC
  AGGTGGACCCCGCAGCAGCACCAGCCCCTCTGGGCCCCATGTGGCACAGAGTGGAAGCATCTCCTTCCCT
  ACTCCCCACTGGGCCTTGCTTACAGAAGAGGCAATGGCTCAGACCAGCTCCCGCATCCCTGTAGTTGCCT
  CCCTGGCCCATGAGTGAGGATGCAGTGCTGGTTTCTGCCCACCTACACCTAGAGCTGTCCCCATCTCCTC
  CAAGGGGTCAGACTGCTAGCCACCTCAGAGGCTCCAAGGGCCCAGTTCCCAGGCCCAGGACAGGAATCAA
  CCCTGTGCTAGCTGAGTTCACCTGCACCGAGACCAGCCCCTAGCCAAGATTCTACTCCTGGGCTCAAGGC
  CTGGCTAGCCCCCAGCCAGCCCACTCCTATGGATAGACAGACCAGTGAGCCCAAGTGGACAAGTTTGGGG
  CCACCCAGGGACCAGAAACAGAGCCTCTGCAGGACACAGCAGATGGGCACCTGGGACCACCTCCACCCAG
  GGCCCTGCCCCAGACGCGCAGAGGCCCGACACAAGGGAGAAGCCAGCCACTTGTGCCAGACCTGAGTGGC
  AGAAAGCAAAAAGTTCCTTTGCTGCTTTAATTTTTAAATTTTCTTACAAAAATTTAGGTGTTTACCAATA
  GTCTTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_007310.2
• Summary: 'Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]'
• Locus ID: 1312