PDE8A (NM_173454) Human 3' UTR Clone

CAT#: SC213479

3`UTR clone of phosphodiesterase 8A (PDE8A) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PDE8A
• Synonyms: HsT19550
• ACCN: NM_173454
• Insert Size: 1269 bp
• Sequence Data:
  Insert Sequence

  >SC213479 3'UTR clone of NM_173454
  The sequence shown below is from the reference sequence of NM_173454. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGGACGAAATGAAGCTGCGGAACCTCCGACCACCTCCTGAATAGTGGGAGACACCACCCAGAGCCCTGA
  AGCTTTGTTCCTTCGGTCATTTGGAATTCCTGAGGGCAGCCAGAGCTCCTTGGTCCTTTCAGTACTAGGC
  AGAACAGCCCCCGATCTGCATAGCCTGTGAAAGCCCACGGGGACATCAGTAACCTTCTGCAGCCACCATC
  CAATGCCATTACTGTCAAGTGAGACTTGGCCACTGTAGCCTGGGCCTGCTGCAGGAGCTCTTCAGAAAGG
  CACATGAGGACCACGGTTTGCCTCAGTTTCTGGTAAAACACAAGGTCTGGAGTGCCCCTGCAAAGGGTAT
  TGATGGACTTCCTGCCAGTGACAGAGCATGTCTATTGCAAACAATTCTCTCAGTTACGTTCAGCACTTAA
  GAACGGCTAATGGCAATAGGATCTTTAGCAACTTTTTCACATCATAGAAGGTGCAATCGCTCACTTGGGA
  ACACTACTGAGAGTGACTTCTCTTTTAAAATTGAGTAGCAGATGAAAAATTAAAATTTGAACTTGATTAT
  TAATATCAATTAAAATGTTTTATTTATTTTATTAAAAGCTCAATATTTTCTATGAATTCAAAAATACTTC
  AGAGCCAAAGCCAACTTCAAATACCGTGACCAAATTTACATGATTCATATTCATTATGCATTACTTGGTA
  TACAGACTTATTTTCATAATGCAAATTAATAAAATGACACTTTTACTGCACTATAGAAATATTCATGTAT
  GTTAAACTTTTCTGATTGAGGCTAACTGGAAAAAGCTGGGGTCGTATTCTAAGTGCTAAAGAAGGCTGCT
  TCTACTGTATAGAACCCAGGGCTCTGAAACAGCTCTAGCCGCCTAATGCACTTCACAGGTAACTCCCCAA
  GGTAAAACTAGACTCTCTTGTTGGTTCGCAAAGAAAAGTTAGGACTTAACACTTTTTTCTAAAATTTTAT
  AATTCAATTTCCAAAAGTCTACTCTATTTTATACTGTTTCTACAAAATATTCCTTATAAAAACAAAGAAC
  AAAAATTGAATATTTAATGAATTGACATTTTATAACCAACCTGTTTTTATCTACGGTGGGAATCTTTGAT
  GCCAGAAATTTATAAAGAGGTTCTGTATCTTCACACCTTGAATAAGCATAATACCATAAAAAATGACACT
  TGACATGTCAATGTATTTGTCATTTCATTTTAAACTCGTATTTGTGGTTTTTTTCCCAGATAAAAATGAA
  ATTAAACCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_173454.1
• Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]'
• Locus ID: 5151