CARD15 (NOD2) (NM_022162) Human 3' UTR Clone

CAT#: SC213503

3`UTR clone of nucleotide-binding oligomerization domain containing 2 (NOD2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NOD2
• Synonyms: ACUG; BLAU; BLAUS; CARD15; CD; CLR16.3; IBD1; NLRC2; NOD2B; PSORAS1; YAOS
• ACCN: NM_022162
• Insert Size: 1276
• Sequence Data:
  Insert Sequence

  >SC213503 3'UTR clone of NM_022162
  The sequence shown below is from the reference sequence of NM_022162. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGACACCAGACTCTTGCTTTGAAGTCTCCGGGAGGATGTTCGTCTCAGTTTGTTTGTGAGCAGGCTGTGA
  GTTTGGGCCCCAGAGGCTGGGTGACATGTGTTGGCAGCCTCTTCAAAATGAGCCCTGTCCTGCCTAAGGC
  TGAACTTGTTTTCTGGGAACACCATAGGTCACCTTTATTCTGGCAGAGGAGGGAGCATCAGTGCCCTCCA
  GGATAGACTTTTCCCAAGCCTACTTTTGCCATTGACTTCTTCCCAAGATTCAATCCCAGGATGTACAAGG
  ACAGCCCCTCCTCCATAGTATGGGACTGGCCTCTGCTGATCCTCCCAGGCTTCCGTGTGGGTCAGTGGGG
  CCCATGGATGTGCTTGTTAACTGAGTGCCTTTTGGTGGAGAGGCCCGGCCTCTCACAAAAGACCCCTTAC
  CACTGCTCTGATGAAGAGGAGTACACAGAACACATAATTCAGGAAGCAGCTTTCCCCATGTCTCGACTCA
  TCCATCCAGGCCATTCCCCGTCTCTGGTTCCTCCCCTCCTCCTGGACTCCTGCACACGCTCCTTCCTCTG
  AGGCTGAAATTCAGAATATTAGTGACCTCAGCTTTGATATTTCACTTACAGCACCCCCAACCCTGGCACC
  CAGGGTGGGAAGGGCTACACCTTAGCCTGCCCTCCTTTCCGGTGTTTAAGACATTTTTGGAAGGGGACAC
  GTGACAGCCGTTTGTTCCCCAAGACATTCTAGGTTTGCAAGAAAAATATGACCACACTCCAGCTGGGATC
  ACATGTGGACTTTTATTTCCAGTGAAATCAGTTACTCTTCAGTTAAGCCTTTGGAAACAGCTCGACTTTA
  AAAAGCTCCAAATGCAGCTTTAAAAAATTAATCTGGGCCAGAATTTCAAACGGCCTCACTAGGCTTCTGG
  TTGATGCCTGTGAACTGAACTCTGACAACAGACTTCTGAAATAGACCCACAAGAGGCAGTTCCATTTCAT
  TTGTGCCAGAATGCTTTAGGATGTACAGTTATGGATTGAAAGTTTACAGGAAAAAAAATTAGGCCGTTCC
  TTCAAAGCAAATGTCTTCCTGGATTATTCAAAATGATGTATGTTGAAGCCTTTGTAAATTGTCAGATGCT
  GTGCAAATGTTATTATTTTAAACATTATGATGTGTGAAAACTGGTTAATATTTATAGGTCACTTTGTTTT
  ACTGTCTTAAGTTTATACTCTTATAGACAACATGGCCGTGAACTTTATGCTGTAAATAATCAGAGGGGAA
  TAAACTGTTGAGTCAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_022162.1
• Summary: This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
• Locus ID: 64127