PLAT (NM_033011) Human 3' UTR Clone

CAT#: SC213631

3`UTR clone of plasminogen activator tissue (PLAT) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PLAT
• Synonyms: T-PA; TPA
• ACCN: NM_033011
• Insert Size: 1278 bp
• Sequence Data:
  Insert Sequence

  >SC213631 3'UTR clone of NM_033011
  The sequence shown below is from the reference sequence of NM_033011. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGATTCGTGACAACATGCGACCGTGACCAGGAACACCCGACTCCTCAAAAGCAAATGAGATCCCGCCTCT
  TCTTCTTCAGAAGACACTGCAAAGGCGCAGTGCTTCTCTACAGACTTCTCCAGACCCACCACACCGCAGA
  AGCGGGACGAGACCCTACAGGAGAGGGAAGAGTGCATTTTCCCAGATACTTCCCATTTTGGAAGTTTTCA
  GGACTTGGTCTGATTTCAGGATACTCTGTCAGATGGGAAGACATGAATGCACACTAGCCTCTCCAGGAAT
  GCCTCCTCCCTGGGCAGAAAGTGGCCATGCCACCCTGTTTTCAGCTAAAGCCCAACCTCCTGACCTGTCA
  CCGTGAGCAGCTTTGGAAACAGGACCACAAAAATGAAAGCATGTCTCAATAGTAAAAGATAACAAGATCT
  TTCAGGAAAGACGGATTGCATTAGAAATAGACAGTATATTTATAGTCACAAGAGCCCAGCAGGGCCTCAA
  AGTTGGGGCAGGCTGGCTGGCCCGTCATGTTCCTCAAAAGCACCCTTGACGTCAAGTCTCCTTCCCCTTT
  CCCCACTCCCTGGCTCTCAGAAGGTATTCCTTTTGTGTACAGTGTGTAAAGTGTAAATCCTTTTTCTTTA
  TAAACTTTAGAGTAGCATGAGAGAATTGTATCATTTGAACAACTAGGCTTCAGCATATTTATAGCAATCC
  ATGTTAGTTTTTACTTTCTGTTGCCACAACCCTGTTTTATACTGTACTTAATAAATTCAGATATATTTTT
  CACAGTTTTTCCAAAATCAGAGTGGAATGGTTTTGTTATAGATGCTGTATCCCACTCTTTATTCATGTTC
  ACATTTTAAAATCATTTGGAATTCTGCTTCACTCGCTTAACATATACACAACACCTGTAACATACAAGGC
  AATGGGCTAGGTGCTCCAGACCGGGAAAAGGAGGGACAGGAATGCTTGGTCTGATGGGCTAATATGGCAT
  TTAGAGAAGTACCAAGGTACAGTGGAGCCGGTCACAAAAGGGCAGACTTGTAGTAGAATTCAGTTGCAAG
  AGGGATTGGGGAATCTTAAGGAAAAAATAGAATCTTAAGGAAAAAATAACTGGGTGAGACGTGGACTGTG
  GACAGGTGTGGAAAAGGCACTCTCCATGGAGGTATGAATATGTAGAGGGCCAAGAGAGGGGAGTACAGGG
  AGAAATGAGTTGAGCTTGTCTGAAGTGAACTTCAGGAAGAGGAACATAGGCTGGAATTTAGATTATGGGG
  GCTCTGAACACCAAACTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_033011.2
• Summary: 'This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]'
• Locus ID: 5327