HGF (NM_001010934) Human 3' UTR Clone

CAT#: SC213672

3`UTR clone of hepatocyte growth factor (hepapoietin A; scatter factor) (HGF) transcript variant 5 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HGF
• Synonyms: DFNB39; F-TCF; HGFB; HPTA; SF
• ACCN: NM_001010934
• Insert Size: 1230 bp
• Sequence Data:
  Insert Sequence

  >SC213672 3'UTR clone of NM_001010934
  The sequence shown below is from the reference sequence of NM_001010934. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACGCTACGAAGTCTGTGACATTCCTCAGTGTTCAGAAGGTAAATAAACCTGAATGCCATGTGGGCCATTC
  TATTCCCCCTATGTGTAGAACTGTAACTCACATTAAAGGTTAACAGCAACGAATCAATCATAACAAATAT
  GTTGTTCGTGCAAATGCAACTACAAATAATTATTTAAACATTTTTATACAATGTTTTTAAAACTGTTGGA
  TTATCACCAGATTAATGCAAAATAACAGAGCGAGTTATCAGTTTGAATTTCAACACTGCCTGAGACATCC
  CTCTGGGGAAAGTGAAAGAGAGGGTTTACTTACCTACTGTCTTGAGCTCACATACCTCAAAATCTACTAC
  TGTGTGGCACCTGAAAGGAGTTGAATGAAGCTTAGCCTTTCATTAGCAATGTTAATTCTATTCAACCAGC
  ACCTGCTTCCACAGAAATTCTGTCCAAACTATCATGAAGTGGTGTGACAAGGGTATATGGACCCAGAAGA
  TAATACAATATAAGAAGGGATCACTGGAAGCTTGACCCCATGCACATTTTGGTGAAAATGTGCCTAGAAT
  CAAATGTGACACGTAGGCTGGAACTGAGTACCATTCAGAATAGGATCTGAAGAGATCAAAGCAATGGAGA
  CCACCAAACTGTCTTGAAGGCATGTCTATGGACCTTAAGTCCATGTCTATGTTTTCAGCTCTTCTCACAG
  CATAAAAGGGCATTGTCCTTACTTTTGCAGTGGAAAACTGAATGGCTGACAAGATGGAAGAGTAACCATT
  TCAGCATTGTATGTGGTTTCATTTTTCTTAGTTATCTGGCTACTGAATAGCCGGATTTTTCAGTTCTGTC
  AGAAACTCTAAATTTCCAAAAATCTAAGTGAAACATGGATGAAACTCTGTTAGAAAATTGTTAGGATTTT
  GGAGTATTTGGGGAGGGGGACTACTGGAATGCTGTCCAAGTTTTATACTAAGATATCTTACCTGTTTGTT
  ATTAACCAAATATTTTTAAAAATATTTCCTCCATAAATATTCATTTAATATTAGGTTGATATTTATCACA
  TAAAAAGTAAAGGCTACTGTTAGCTAATTGTCACAGAGAAGGATTTGTTTTCTGTTGTTAGTGAATTTGA
  AATCCTTGACTTTATGTGCTACAGCCAGTTCCATCTCTGTTTGTAAATTCTTACTTTCCATTCCATATCA
  TATTCTGTTCCCTATAACCTCTTCATTGTTTTCTTTTCTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001010934.1
• Summary: 'This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. [provided by RefSeq, Nov 2015]'
• Locus ID: 3082