GM130 (GOLGA2) (NM_004486) Human 3' UTR Clone

CAT#: SC213684

3`UTR clone of golgi autoantigen golgin subfamily a 2 (GOLGA2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GOLGA2
• Synonyms: GM130
• ACCN: NM_004486
• Insert Size: 1273 bp
• Sequence Data:
  Insert Sequence

  >SC213684 3'UTR clone of NM_004486
  The sequence shown below is from the reference sequence of NM_004486. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGACGAGAATGATGAGGTGAAGATCACTGTCATCTAAAAGCCGGCTACTGTCAGCAAAGCCTGAAGAAG
  TGGGGCTGGATACCCTGCCCCCACCATATCCCTACCATCCCTTCTCAGTCAACCCTTTACCCTTACAGTA
  GCAAGCATAGACCCCTGTCTAACGGGGGTAGACAGGTGCAGATGAGGTGAAGATCACTGTCATCTAAAAG
  CTGGCCACTAAATTAAAAAAAAATTAAAAGTTATGGGATTAAAAAAAGTTATGGGATAAAAAAGGTTATG
  GGTAACTTATGGTATAAAAGTTATGAAAAAAGGCCAGGTGCAGTGGCTCACGCCTGTAATCCCAGCACTT
  TTGGGAGGCTGAGGTTGGGAGTTCAAGACCAGCCTGGTCAACATGGTGAAACTGTGTCTCTACTAAAAAT
  ACAAAAATTAGCCAGGCATGGTGGCACGTGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAAT
  CGCTTGAACCTGGGAGGTGGAGGTTGCAGTGGGCTGAGATCACGCCATTGCACTCCAGCCTGGGCGACAG
  AGTGAGACTCTGTCTCAAAAAAAAAAAAAATTATGAAAAAAGTTATGGGATTAAAGAAAGTCAGGATAAA
  AATTTTAAAAAGCAGGCCACTGTCAGCAAAGCCTGGAGAAGTGGGGCCGGAGGCTCCGCCCCCATCATGT
  GCCTGCCACCCCTTCCCAGTCATCCCTTTACTCTTACAGTAGCAAATAAGACCCCTGTCTAATGGGGGGA
  GACAAATGTGTAGACCCTTAGCCACCTTGGCCAGGGCTGACTCCTTAAATTTCTGGATGATGATGATTGT
  TATTTAATAGCCAGAGGCTCATATAATTGGCCTCTTTGGAAGAGGCCTCATGGCCTCCTTACTCTCACCA
  AAGCAATTTTTCCCTCAGGGGGGCTCCCATCTTCTTACACAGAGAGGCAGCTGAGGCAGGACAGTGGGGC
  TAACTGTAGACCAGGCGAGGGCACGGGCTGCTGGGGTGGCCCTGCTTCCCCAGTGTACATATTGTATCTG
  TGTAACATTTTGTATATTCCAGGGGTAGGGCCGCCCCCTGTATCATACCTAGCAGAGGTTGGAGCTGGCA
  CATGGGGAGGAGGTTCTAATAATTATTTGGGGCTGGGAAACTTATTTATTGATAGCATAGGACAGAGGAA
  GGAGGCGGGGATGGGGTCGTGGCGCCCTGGTGATGCGACTCCTGTTTATTTTGCTTTTTATTTCGGAATA
  AATGGATTTAGCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004486.4
• Summary: 'The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010]'
• Locus ID: 2801