SREBP1 (SREBF1) (NM_001005291) Human 3' UTR Clone

CAT#: SC213707

3`UTR clone of sterol regulatory element binding transcription factor 1 (SREBF1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SREBF1
• Synonyms: bHLHd1; SREBP1
• ACCN: NM_001005291
• Insert Size: 1267 bp
• Sequence Data:
  Insert Sequence

  >SC213707 3'UTR clone of NM_001005291
  The sequence shown below is from the reference sequence of NM_001005291. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACCACTGTCACTTCCAGCTAGACCCCGTGTCCCCGGCCTCAGCACCCCTGTCTCTAGCCACTTTGGTCCC
  GTGCAGCTTCTGTCCTGCGTCGAAGCTTTGAAGGCCGAAGGCAGTGCAAGAGACTCTGGCCTCCACAGTT
  CGACCTGCGGCTGCTGTGTGCCTTCGCGGTGGAAGGCCCGAGGGGCGCGATCTTGACCCTAAGACCGGCG
  GCCATGATGGTGCTGACCTCTGGTGGCCGATCGGGGCACTGCAGGGGCCGAGCCATTTTGGGGGGCCCCC
  CTCCTTGCTCTGCAGGCACCTTAGTGGCTTTTTTCCTCCTGTGTACAGGGAAGAGAGGGGTACATTTCCC
  TGTGCTGACGGAAGCCAACTTGGCTTTCCCGGACTGCAAGCAGGGCTCTGCCCCAGAGGCCTCTCTCTCC
  GTCGTGGGAGAGAGACGTGTACATAGTGTAGGTCAGCGTGCTTAGCCTCCTGACCTGAGGCTCCTGTGCT
  ACTTTGCCTTTTGCAAACTTTATTTTCATAGATTGAGAAGTTTTGTACAGAGAATTAAAAATGAAATTAT
  TTATAATCTGGGTTTTGTGTCTTCAGCTGATGGATGTGCTGACTAGTGAGAGTGCTTGGGCCCTCCCCCA
  GCACCTAGGGAAAGGCTTCCCCTCCCCCTCCGGCCACAAGGTACACAACTTTTAACTTAGCTCTTCCCGA
  TGTTTGTTTGTTAGTGGGAGGAGTGGGGAGGGCTGGCTGTATGGCCTCCAGCCTACCTGTTCCCCCTGCT
  CCCAGGGCACATGGTTGGGCTGTGTCAACCCTTAGGGCCTCCATGGGGTCAGTTGTCCCTTCTCACCTCC
  CAGCTCTGTCCCCATCAGGTCCCTGGGTGGCACGGGAGGATGGACTGACTTCCAGGACCTGTTGTGTGAC
  AGGAGCTACAGCTTGGGTCTCCCTGCAAGAAGTCTGGCACGTCTCACCTCCCCCATCCCGGCCCCTGGTC
  ATCTCACAGCAAAGAAGCCTCCTCCCTCCCGACCTGCCGCCACACTGGAGAGGGGGCACAGGGGCGGGGG
  AGGTTTCCTGTTCTGTGAAAGGCCGACTCCCTGACTCCATTCATGCCCCCCCCCCCAGCCCCTCCCTTCA
  TTCCCATTCCCCAACCTAAAGCCTGGCCCGGCTCCCAGCTGAATCTGGTCGGAATCCACGGGCTGCAGAT
  TTTCCAAAACAATCGTTGTATCTTTATTGACTTTTTTTTTTTTTTTTTTCTGAATGCAATGACTGTTTTT
  TACTCTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001005291.2
• Summary: 'This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017]'
• Locus ID: 6720