Pyruvate Kinase (PKLR) (NM_000298) Human 3' UTR Clone

CAT#: SC213725

3`UTR clone of pyruvate kinase liver and RBC (PKLR) nuclear gene encoding mitochondrial protein transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PKLR
• Synonyms: PK1; PKL; PKRL; RPK
• ACCN: NM_000298
• Insert Size: 1297 bp
• Sequence Data:
  Insert Sequence

  >SC213725 3'UTR clone of NM_000298
  The sequence shown below is from the reference sequence of NM_000298. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCTACACCAACATCATGCGGGTGCTAAGCATATCCTGAGACGCCCCTCCCTCCTCTGGCCCAGCCTACCC
  TTGTACCCCATCCCTTCCTCCCCAGTCTACGTTCTCCAGCCCACACCCCTCCAAAGCCCCACCTTTAAGT
  CCTCTCTTCTCTATTCCTGACCCTCCCTACCTGAGGCCTATCTGAGACTATAACTGTCATCTAGCCCCTT
  CGAGGTTGCCCCTTCCCCATCTCCATTTCACACAGGTCCTGAAAGTCTGTGTCCAATTATGCACTGGCCA
  CCCAACAGCACCAATTGTACATTCCCTGCATCCAATCTGCTCAGCAGGCCCTAAGATGCCTTGAGTCTTT
  AATCCCAGTTTGGCTGGTTAATTCCATAACCCCAGGCATCCCATCCCTTGGGGTGGGGGAGAGGGGAGAC
  AGGGCAATCTTGTCCACAGTCTCCCATTCTCATATGTAGCCCTCATGATAATCTGGGCATCTCGTGCCAG
  GGCAGGCTACCCCTTCATGGTGACTAACAGTTACATGAAAGTCCACGCTTTTGGGAAAACTGGGTGGGAT
  GGATGCTGGGGAGAAGTGAGGGCTGGGCAGCTGATTTTGTCACTGTCTTCACAACCTCCGTGCTGGGCTT
  GTAGACCACTGTCCTGGCTGCTCTCATGCCTGCCTGATACCCTGCTTGGTCAAATCCCCGGCTGCTTCCT
  TCTGCACCCAGAAATTCCTTCCCACTCATGTTGTTCCCACACACAAACCAAGAGCCAAAAATGAGTGTGT
  CTATTTTATATTTAAACCCAGCTGTTTGGAAGCAATTATAAAACTTCTCCCACACACCAAGAACCCCAGA
  ACTCCCCACCCAGAAGGAAAGGAGACTTAGGGTCCTGGTCCCACAGTCTTTAATGCTGTAGAGTAGGAGA
  GGGGACAGGCAGAGTGAGGGTGGTAAAGGACTACTCTTGTCCCTGAGAAGGCAGAGGTGCTGGCTGGCCT
  GCCCTTCCCCAGGCTTGGATACCTTGGGCCTTTCCCTTATTCCTGCACCAACAGCAAACTCAGAAGGAAA
  AAACAAAACAAAACTCTAAAGGTAAACGCGGTCCTTCTCCCTCTCTCATGTGGCTCCTCCTGCCCCTACC
  TGGGAGAAGGTTCAAGTATTGCGCTGATGGGTTTGGGCAAGGACCACAGAGGCTGGCATGGAGAGGCCCT
  GCCTCCTCCTACTCCTCCTGTCTCCTTACCTGAAAAGGAGGGGAGGGATGGATAAAGGAATGGGTAAACA
  AAATTAATTGGTAACACATAAAGTTAGTAAATGAATA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000298.5
• Summary: 'The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 5313