ErbB 3 (ERBB3) (NM_001982) Human 3' UTR Clone

CAT#: SC213727

3`UTR clone of v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) (ERBB3) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ERBB3
• Synonyms: c-erbB-3; c-erbB3; ErbB-3; erbB3-S; FERLK; HER3; LCCS2; MDA-BF-1; p45-sErbB3; p85-sErbB3; p180-ErbB3
• ACCN: NM_001982
• Insert Size: 1329 bp
• Sequence Data:
  Insert Sequence

  >SC213727 3'UTR clone of NM_001982
  The sequence shown below is from the reference sequence of NM_001982. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGGCATAGCAGGCTTTTCCCCAAGGCTAATGCCCAGAGAACGTAACTCCTGCTCCCTGTGGCACTCAGGG
  AGCATTTAATGGCAGCTAGTGCCTTTAGAGGGTACCGTCTTCTCCCTATTCCCTCTCTCTCCCAGGTCCC
  AGCCCCTTTTCCCCAGTCCCAGACAATTCCATTCAATCTTTGGAGGCTTTTAAACATTTTGACACAAAAT
  TCTTATGGTATGTAGCCAGCTGTGCACTTTCTTCTCTTTCCCAACCCCAGGAAAGGTTTTCCTTATTTTG
  TGTGCTTTCCCAGTCCCATTCCTCAGCTTCTTCACAGGCACTCCTGGAGATATGAAGGATTACTCTCCAT
  ATCCCTTCCTCTCAGGCTCTTGACTACTTGGAACTAGGCTCTTATGTGTGCCTTTGTTTCCCATCAGACT
  GTCAAGAAGAGGAAAGGGAGGAAACCTAGCAGAGGAAAGTGTAATTTTGGTTTATGACTCTTAACCCCCT
  AGAAAGACAGAAGCTTAAAATCTGTGAAGAAAGAGGTTAGGAGTAGATATTGATTACTATCATAATTCAG
  CACTTAACTATGAGCCAGGCATCATACTAAACTTCACCTACATTATCTCACTTAGTCCTTTATCATCCTT
  AAAACAATTCTGTGACATACATATTATCTCATTTTACACAAAGGGAAGTCGGGCATGGTGGCTCATGCCT
  GTAATCTCAGCACTTTGGGAGGCTGAGGCAGAAGGATTACCTGAGGCAAGGAGTTTGAGACCAGCTTAGC
  CAACATAGTAAGACCCCCATCTCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAACTTTAGAACTGGGTGC
  AGTGGCTCATGCCTGTAATCCCAGCCAGCACTTTGGGAGGCTGAGATGGGAAGATCACTTGAGCCCAGAA
  TTAGAGATAAGCCTATGGAAACATAGCAAGACACTGTCTCTACAGGGGAAAAAAAAAAAAGAAACTGAGC
  CTTAAAGAGATGAAATAAATTAAGCAGTAGATCCAGGATGCAAAATCCTCCCAATTCCTGTGCATGTGCT
  CTTATTGTAAGGTGCCAAGAAAAACTGATTTAAGTTACAGCCCTTGTTTAAGGGGCACTGTTTCTTGTTT
  TTGCACTGAATCAAGTCTAACCCCAACAGCCACATCCTCCTATACCTAGACATCTCATCTCAGGAAGTGG
  TGGTGGGGGTAGTCAGAAGGAAAAATAACTGGACATCTTTGTGTAAACCATAATCCACATGTGCCGTAAA
  TGATCTTCACTCCTTATCCGAGGGCAAATTCACAAGGATCCCCAAGATCCACTTTTAGAAGCCATTCTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001982.2
• Summary: 'This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]'
• Locus ID: 2065