DUT (NM_001948) Human 3' UTR Clone

CAT#: SC213761

3`UTR clone of deoxyuridine triphosphatase (DUT) nuclear gene encoding mitochondrial protein transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DUT
• Synonyms: dUTPase
• ACCN: NM_001948
• Insert Size: 1316 bp
• Sequence Data:
  Insert Sequence

  >SC213761 3'UTR clone of NM_001948
  The sequence shown below is from the reference sequence of NM_001948. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAGGAGGTTTTGGTTCCACTGGAAAGAATTAAAATTTATGCCAAGAACAGAAAACAAGAAGTCATACCTT
  TTTCTTAAAAAAAAAAAAAAAGTTTTTGCTTCAAGTGTTTTGGTGTTTTGCACTTCTGTAAACTTACTAG
  CTTTACCTTCTAAAAGTACTGCATTTTTTACTTTTTTTTATGATCAAGGAAAAGATCATTAAAAAAAAAC
  ACAAAGAAGTTTTTCTTTGTGTTTGGATCAAAAAGAAACTTTGTTTTTCCGCAATTGAAGGTTGTATGTA
  AATCTGCTTTGTGGTGACCTGATGTAAACAGTGTCTTCTTAAAATCAAATGTAAATCAATTACAGATTAA
  AAAAAAAAGCCTGTATTTAACTCATATGATCTCCCTTCAGCAACTTATTTTGCTTTAATTGCTTTAAATC
  TTAAGCAATATTTTTTATTCAGTAAACAAATTCTTTCACAAGGTACAAAATCTTGCATAAGCTGAACTAA
  AATAAAAATGAAAAGGAGAGATTAAAGGTATTCCTTGTTCTTCCCTTCTCTTCACTAGTCTAAAAACTTC
  TTTTTAATCTTAAGATTCTTTGTGATGAGGGTGAGAAAAAGAATCCTCAGTTTATTTTTCCACTATTAAT
  CTTTCTTTTGATAAATCCTCTATTGACTGGGTAGAGGTATGTTTGTGAAAGACATGTAACTTGGGGATTT
  GTTACTTTAGGTTTGTTCCCTTGAATTTCATCTCATCAGGCAAATTGTACTAGTTGTAGTTACGAGTTTT
  CCCTCAGTGAAGTAGCAATAGGCTGTAATCAAGAAAATATGCCATTTATAGAGATAAGATAAATGAAATA
  ATACTTCAGCCACCAGGTTTTTCTGTCTCACATACATAAGCAGCATTTCATTGCAGATATGGGACTGATT
  CTGTGGCTTACCTTGATTAACATCTTTTGGAAGTTTTGCTAGTGTGCTTTCCTTTCTTTACTATGTTTCT
  CAGATTCCTTTGTATCAGGGTTTTGGGTGTCACTTAGGTTTTGTCCATCAGATTCTGTGAGACACCAGGC
  ATCGTTTTGAGGATGTGGGTTATACACATGGAGTGCTTCTGGAACTATCAGCCCACTTGACCACCCAGTT
  TGTGGAAGCACAGGCAAGAGTGTTCTTTTCTGGTGATTCTCCAGGCCATTTAATACCCTGCAATGTAATT
  GTCCCTCTGTGGCTCACATTTCATTAGTGAGCCATGAAATCAACTCAGTGGGACATAGCCAGCATTTTTG
  CATACCAGGTTGGGCTATAAAATATTTCTGTTGTCAATAAATTTTAAATGTTTTCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001948.3
• Summary: 'This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]'
• Locus ID: 1854