ATP6V1B2 (NM_001693) Human 3' UTR Clone

CAT#: SC213825

3`UTR clone of ATPase H+ transporting lysosomal 56/58kDa V1 subunit B2 (ATP6V1B2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ATP6V1B2
• Synonyms: ATP6B1B2; ATP6B2; DOOD; HO57; VATB; Vma2; VPP3; ZLS2
• ACCN: NM_001693
• Insert Size: 1304 bp
• Sequence Data:
  Insert Sequence

  >SC213825 3'UTR clone of NM_001693
  The sequence shown below is from the reference sequence of NM_001693. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTCAGCGAATTTTACCCTCGAGACTCTGCAAAGCATTAGCTGCTGCTTCTGCATTGCTCCGCGCTCTTGT
  GAAATACTGGTTCTGTTTTCTTTATTCCTTTTGCACTCTCGGTTCCCACCTTTGTGTTGGAGTTTACCAT
  GTTACCCTGTAATTAAAAACAAAGAATAGGTAACATATTGTGCCAGTGTTGCAACGTTTTAAACTGCTAA
  CAGACCTTAAAATATCCCCCTACCTGGGTCCTCAGTGCTATGTTTAAAGTGCTGCAGGGATGGAGTGGCG
  TTTTCTTATTGCTGTATGTATTGTACATAGTGGAGTAGTTAGTTACCTGATAACAGTCTTGTTATTTGGG
  TCTCTTAGACCTTACCTCTCAACTCCCTCAAGAGTACCAGTCTCTGAAGTTATAATGCTTTGGTCTCTAC
  ATTAGGGGCAAGATCCAGTCTGAGAGAAGTCTCCTTTGAGAAGGGCCAAGAGGCTCTTTCCTGAGTGTTT
  GCTTTCGGTTTGTTGGTATGCCTGTATTGCTGGGCTGTGCTGCTGCTCGAAGCAGATGGTTTTGACTGTC
  TTTTTGCTCTTTCCTATATAATGAATAGATGAGTGAAAGGAGTTTTCTTTTTCTCTTTAGTACTTACGTA
  TTGGGATTCCTGTGTCTTACAGCTCTCCCTCTCCAAATAATACACAGAATCCTGCAACTTTTTGCACAGC
  TGGTATCTGTCTGGTAGCAGTGAGACCCCTTGTCTTGGTGATCCTTACTGGGTTTCCAAGCAGAGGAGTC
  ACATGATTACAATTGCCAGTAGAGTTGTTGTTTGGGGTACAAGATGAGAAGAAAGAAAAACCTACAGCCT
  TTCTACATTCTGACATGCTAACAGTGGTTTAAGTTTCTAAAGTGTTTACCAGATGCTGAAGGCAAGGGGA
  GGGAGCAGAAGCACTTATGTTTACGGATATTTTAAACTCTGTTAGAGAGCAGCCTTTGAAAATCCCCAAT
  TTGGTTCTGCTTTTTGACCTCTCTCTACCTTTTCAGGGTAATCTTTGTGGCACAAACGATAGCATTTCCA
  AGCTTTAGAGTTTTCTGAATTCCTGCGCCTTCCTGACGTGAGCCCTGAGCGATCTTCTATGCAGTTCTGC
  CATGCGTCCTGTTGGTCTCTCTGTGTTCTTTGTTACTTGGGTGCAATAGCAACTTCCCTACCCCGTGCAT
  TCCATCTTTCATGTTGTGTAAAGTTCTTCACTTTTTTCTCTGAGGGCTGGGGGTTGGGGGAGTCAGCATG
  ATTATATTTTAATGTAGAAAATGTGACATCTGGATATAAAATGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001693.3
• Summary: 'This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008]'
• Locus ID: 526