Adducin 2 (ADD2) (NM_001617) Human 3' UTR Clone

CAT#: SC213860

3`UTR clone of adducin 2 (beta) (ADD2) transcript variant beta-1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ADD2
• Synonyms: ADDB
• ACCN: NM_001617
• Insert Size: 1290 bp
• Sequence Data:
  Insert Sequence

  >SC213860 3'UTR clone of NM_001617
  The sequence shown below is from the reference sequence of NM_001617. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGAGAAAGTGGAGTCCTGATTCATGACACCCTTGGGCTCCCTCCTGCCTCCTCTCTCTCCTCCCCTTCCC
  TTCTCCCATCTCTGTCCCTGCAAGCACAGGGCTAAGGAGGGATAGAGTAGGACCCTGGACCACATTCGGA
  AGGGGAACTTAGAGATCACCCGACCAACCCTTCGTTTTACAGTTGCCCAAGAGAAATCAGGTGACTTGCC
  CAAGGTCACACAGCTAGTTAGCGGCAGAGCCTGCACTCGAATTCAGGTCTCCTGACTTCCAGTCCAGTGC
  TCCTTCTACTACACAACACTGCCTAGTTGTGGGCTGCCTTTGTTTGGATGCTGTCCACCAATCTGAGCCT
  AGGGCAAGAAGGCCAGAAATGGGCCGTGAGCTCTCACAGGCTCAGACTAAATCAGAGGTCAAGGCTTCCC
  CTGAGTAAGGTCCATTTCTTCCCAGGAATCCAATCTCCTGTGGATGGAGCTATCTCTACATTTAAAAATC
  TCTTCTCTTTTCCACTTTGGGTCCCTGCCCTGCTGCTCAAAGTGACTAGCCAAATTGACCCCTCCAACAG
  AAAGTAATCTTTGTTCCCAAGGGCTGATGGCTTAGCTTGTACTACCCCAAACATTAACCCTGAGCTTTCT
  TCATGGAACCTCTTGAATGATGGATGGAAGAGCTATAAGAGGTGGTAGGCATAGGGGCAAGCCATGTAAG
  CTGAGGATTGGGGATGGTTTCATCAACATAAGAGGCCAGGAACTTGACCCCTTTGAATTGTGCATCTCAG
  GCACTTCAAAACTAAAACCAAATTTAGCATAGGAAAAAGTTGTTTAATGCTCAGGGCAGAAATTTGGGGA
  AGTTGAAATCCTCTGTTGGCTTTGGGTTGTATAAGGAGGATCAAAACAACAGAGGAAATGCTGACTTTCT
  AGCTTTGCATGACACCTGGAGCAATGCACTGTACCTGCCTCACTCCTGTCCAGTGGTCAGGTTTCCCCTG
  ACCTTCCCTCACCCCCAGAAACACTTGCTTACAGACCGAAACTGGCATCTTACTCTTGGCACCTTGACTT
  GCACCCTCTGAGGTTCCAACTCAGTCATTCTTTGTCCAGCAGAGGAGAATCAGAAATGAGCCCTTCAGGA
  TTAATCCTCTTGCACCAGCTCTCAGAGAAATGCTGGGTATCCCTGTCCTTGTCCCTATCTGTCCATCCTG
  GGGCCTGGTAATGGCCACAGTTATTGTTTTAAATGCCAACACTGTCTTCTCATGTTCTTCCGTGGGGCAT
  TGATTAATGAGCATTTGTTGGCTCCTAAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001617.2
• Summary: 'Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010]'
• Locus ID: 119