Sorbitol Dehydrogenase (SORD) (NM_003104) Human 3' UTR Clone

CAT#: SC213863

3`UTR clone of sorbitol dehydrogenase (SORD) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SORD
• Synonyms: HEL-S-95n; RDH; SDH; SORD1; XDH
• ACCN: NM_003104
• Insert Size: 1299 bp
• Sequence Data:
  Insert Sequence

  >SC213863 3'UTR clone of NM_003104
  The sequence shown below is from the reference sequence of NM_003104. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGTGACCAGAATCCCTGATGTTAATGGGCTCTGCCCTCATCCCCACAGTCTTGGGATCTCAGGGCACAAT
  GGCTGGACATGGGTGGGCTCTGATGCAGAACTTTCTCTTTTGAATGTTAAGAATAACTAATACAATTCAT
  TGTGAACAGAAGTCCTTAAGCAGAGGAATTGGTGTGCCTTAAAGATACAATCTGGGATAGTTTGGGGGAA
  CTTGTAGCCAGAATGCCCTGTTCATGCTGAGCAAAGTTCAGCAAGTAGAGCAGAGTTTGGCAGGCAGGTG
  CCAGGAACTCCCCTTCTTCCTGGAGTGCCTTCATTGAGGAAGGAAATCTGGCCCTTGGGTTTCCTGGTTC
  CACTGCTACTGACCCAGAGGGGAATGAGGGCTGAGTTATGAAAAGATAACTTCATGAAGACTTAACTGGC
  CCAGAAGCTGATTTTCATGAAAATCTGCCACTCAGGGTCTGGGATGAAGGCTTGTCAGCACTTCCAGTTT
  AGAACGCAATGTTTCTAGAGACATATTGGCTGTTTGTTTTGATGATAAAAGGAGAATAAGAAAAGGCATC
  ACTTTCCTGGATCCAGGATAATTTTTAAACCAATCAAATGAAAAAAACAAACAAACAAAAAAGGAAATGT
  CATGTGAGGTTAAACCAGTTTGCATTCCCCTAATGTGGAAAAAGTAAGAGGACTACTCAGCACTGTTTGA
  AGATTGCCTCTTCTACAGCTTCTGAGAATTGTGTTATTTCACTTGCCAAGTGAAGGACCCCCTCCCCAAC
  ATGCCCCAGCCCACCCCTAAGCATGGTCCCTTGTCACCAGGCAACCAGGAAACTGCTACTTGTGGACCTC
  ACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGGACTTCCCCCACCCCAGAAGATTAGCATCCCATACTA
  GACTCATACTCAACTCAACTAGGCTCATACTCAATTGATGGTTATTAGACAATTCCATTTCTTTCTGGTT
  ATTATAAACAGAAAATCTTTCCTCTTCTCATTACCAGTAAAGGCTCTTGGTATCTTTCTGTTGGAATGAT
  TTCTATGAACTTGTCTTATTTTAATGGTGGGTTTTTTTTCTGGTAAGATTTAGACCTAAATCGCATCATG
  CCAACTTGTGACTTTGAGACTATTCATCAAGAATGAGGATATAGTAGCCATGACATAGCTTGAGCTATAG
  CCTTTAATTCCTTACTTTGGCTATGGGTGGAGGGTGAGTTTGAAGAGGTTCTGATTTTCTTGTAACCTGG
  GAAAGCCATGACCTTGTGCCCGATTCTTTCAGATTGCTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003104.4
• Summary: 'Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]'
• Locus ID: 6652