Bestrophin 3 (BEST3) (NM_032735) Human 3' UTR Clone

CAT#: SC213892

3`UTR clone of bestrophin 3 (BEST3) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: BEST3
• Synonyms: VMD2L3
• ACCN: NM_032735
• Insert Size: 1304
• Sequence Data:
  Insert Sequence

  >SC213892 3'UTR clone of NM_032735
  The sequence shown below is from the reference sequence of NM_032735. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCTGAACAAGGAAACTGAGGAATCACCCAAATGAGTGCCACCAAGTTCTAGGACCTGGTTCTAGCCTAGA
  TTCTTACCTCCCCAAAAGCAGCATATTAGGCCCAGGACATACCTGAAGGCTGGTCGACTTTTTAAAAAAC
  ATGATCACACTGTATAAGCTACTTAGAACTTTGAAGGGCATTATGATCCTGACTTTTTGAGAGCTGCGAA
  AAATCAAACGCATTCACGTCATCCAACACAACATGGCTTTCATCAGAAGTTACACAGACCACAGTGATTT
  AGCCAAATAAGTCTTAACCAAAAAAAGGAATCTGAAAAACATACATGACTTATAAATTCATAGATTATTA
  GTTGTGGAAAGAGTCTCATAGATCATAGTCCAATCCCCTAGACCCAAAGGCCCAGACACGCAGGGTTCTC
  TATTTCTCTTCTTTTTTCTCTTTCTTTTTTTCTTTAGCCATAAAGAAAGGAGATGGATCACCTAATGCCT
  GAAAGATCCCCTCCTTCCTAACGTTTTTGTTTGAGGCTTTCTAACCATCTCATTCCTACTTTTATAACTA
  TTATTGTTATTATCACTTCCTGATTCTTAAGCTTCATCAGCACCAGAACACTTAATTCTCTTCAATTTCC
  TTTTTTACTTTTCTCTCTTTTCCTTTTATTCTGTCATCCTTTTTTTCCCTTCCCTTCTCCCTGGTTGTGC
  TGCTTTTCTAGTAATCTGTCCCATTTTTCAGGTTATAATATTTCCTCTTGCCAAATCTGTTGCTTATGTC
  TTGCCATGAATTTCAAAATATAAGACATTGGCAAAGCCTCCTACAGAATCATCAAAGGTGTCAACAAATC
  CAGCCTGATACCCTTCCAGTCTCACTTCCAGTGGAAGGCATATAGGAGTGATGGTTCCCCCCGTGCCCTT
  GCAGCCATTGACATTTCTTTCTGCTTCCACTATTTGGTGTTGGGCACTGGAGAAAAACGAAGCCAAGAGA
  ATATGAAATTTGCATAGCCAAATGCTATGGAACCAAAGAATAAGATGACTCACATCTGTTTAACTTGAAC
  TCTTATGCAAAAATCCTAATCGTGTCGCAACACGGGACCAGTTACTGTGTCTATCGGGTCATTTGGGAAT
  GGGCAGGATACTGGAGCTGGGAGGCTAATGGGGTCCAGAGCTGCATAATGGGGATCGGATGTTGGTACTC
  CTCCCAGGGAGCTCTCAGGACAGCTGTTTGTCGTGGGCTCCCAAGTGCCTAGGCCATGCCTTACGACTGC
  TGCACTTACCATTTGTCATATGATGGCACTTGACTACATATGGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_032735.2
• Summary: BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]). [supplied by OMIM, Mar 2008]
• Locus ID: 144453