CTCF (NM_006565) Human 3' UTR Clone

CAT#: SC213937

3`UTR clone of CCCTC-binding factor (zinc finger protein) (CTCF) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CTCF
• Synonyms: MRD21
• ACCN: NM_006565
• Insert Size: 1342
• Sequence Data:
  Insert Sequence

  >SC213937 3'UTR clone of NM_006565
  The sequence shown below is from the reference sequence of NM_006565. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCGAGATGATCCTCAGCATGATGGACCGGTGATGGCGGAGCCTTGTGCGTCGCCAGGACTTCTCTGGGCT
  GTGTTTAAACGGCCCGCATCTTAATTTTTCTCCCTTCTTTCTTTTTTTGGCTTTGGGAAAAGCATCATTT
  TACCAAACATACCGAGAACGAAAACTTCAAGGATGATGTTAGAAAAAAATGTGATTTAACTAGAACTTGC
  TGTCTGATGTTAGCAAATCATGGAATGTTCTGAGTCCCTGAGGGTTTACTGTGAAGTGCTGAGGACAGTG
  TTGACAACTAACTCGTTTTCCTAGATGGAAACGGAGACATTGACCCCTCCCTCCATGTGGTAAACCACTC
  CAGAATGGCCACCAGGCTTCCCAGAGTTCTATGGTCTTCTTCCCAAGAGAGTTTTTAATTGTAAATGCAT
  ACTTGGGAAGGACTTAGAGTTTTAAACTGTTTTTTGCTTTTGCTTTTCCCTGACTCCCTTTGCTTGGAGT
  CAGCTGCACACCAGTAGTATGGCATGCTACGATCAGGTTCTGTCCTGAAAGCTTTGCCTCTTTCTTGGCA
  AAGTTTCTGGTATGGTCAAGCTTGTAAATAACTTTTTTTACATTTTAATCTTTTCCATTAATTAAGAGGT
  TGAAAAGAAGTGCAGTGTAAGAAAACCCAGCATTTTAATTACTTGCAAATTAAGTTACCACAGACTCTGT
  AGTGTGTAAATGTTGACAAGGAATTGGATCACAATCATGTAGCAGAATGGCACCCAGACCACTGCCCACC
  AGTGACGGACATGCACGTGGCAGATCATGATTTCCAGCCCACGGAGCCAGCATTTGAACCTTGTATAATT
  AACTTTCAGTTATGATTTCCCATCGACATTTTCTTTGCCCTGTTTGTAGCTGATTGTTGTGTTTTATAAA
  TCTTCTGTTAAGGCAGAAGGGTGATTATGAGTGGTTCACAGCAGCCCTTATAAGCTGGGCCAGAAAATTT
  CACTAGGTCAGTAATTTAAACCTTGGATCTTCAAAAAATAAAATAATGTGAAGCAAAACCAACTAAAAAG
  TGATTCTTGCACATGAACTGTCACATGTTTAAAAATGTGTTTTTTAGAGAGCCTCAGTCTTACTGATTTC
  AAACACTTTTTTCTTCTGTGTATTGCTTTTAAGAGAGCCATCAGTTAGCTATCAGACTCTAGGTTGATGC
  ATTTTGTACTTAGCTGTACTGTGTGATATTTTTCATTATTTTAGGACGCCAACATGAGACCTGTAATAAA
  ATATGTAATGGGGTTGAAAGCTGGGGAGGAGGATCTACTGCTGTACAGCTAATAAATCATAACGGATTAA
  CAAGTGCTCCAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_006565.2
• Summary: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
• Locus ID: 10664