PACE4 (PCSK6) (NM_138319) Human 3' UTR Clone

CAT#: SC214014

3`UTR clone of proprotein convertase subtilisin/kexin type 6 (PCSK6) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PCSK6
• Synonyms: PACE4; SPC4
• ACCN: NM_138319
• Insert Size: 1341
• Sequence Data:
  Insert Sequence

  >SC214014 3'UTR clone of NM_138319
  The sequence shown below is from the reference sequence of NM_138319. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CATTCAGTTCTGCTGCCGCACGTGCCTCCTGGCCGGGTAAGGGTGCCTAGCTGCCCACAGAGGGCAGGCA
  CTCCCATCCATCCATCCGTCCACCTTCCTCCAGACTGTCGGCCAGAGTCTGTTTCAGGAGCGGCGCCCTG
  CACCTGACAGCTTTATCTCCCCAGGAGCAGCATCTCTGAGCACCCAAGCCAGGTGGGTGGTGGCTCTTAA
  GGAGGTGTTCCTAAAATGGTGATATCCTCTCAAATGCTGCTTGTTGGCTCCAGTCTTCCGACAAACTAAC
  AGGAACAAAATGAATTCTGGGAATCCACAGCTCTGGCTTTGGAGCAGCTTCTGGGACCATAAGTTTACTG
  AATCTTCAAGACCAAAGCAGAAAAGAAAGGCGCTTGGCATCACACATCACTCTTCTCCCCGTGCTTTTCT
  GCGGCTGTGTAGTAAATCTCCCCGGCCCAGCTGGCGAACCCTGGGCCATCCTCACATGTGACAAAGGGCC
  AGCAGTCTACCTGCTCGTTGCCTGCCACTGAGCAGTCTGGGGACGGTTTGGTCAGACTATAAATAAGATA
  GGTTTGAGGGCATAAAATGTATGACCACTGGGGCCGGAGTATCTATTTCTACATAGTCAGCTACTTCTGA
  AACTGCAGCAGTGGCTTAGAAAGTCCAATTCCAAAGCCAGACCAGAAGATTCTATCCCCCGCAGCGCTCT
  CCTTTGAGCAAGCCGAGCTCTCCTTGTTACCGTGTTCTGTCTGTGTCTTCAGGAGTCTCATGGCCTGAAC
  GACCACCTCGACCTGATGCAGAGCCTTCTGAGGAGAGGCAACAGGAGGCATTCTGTGGCCAGCCAAAAGG
  TACCCCGATGGCCAAGCAATTCCTCTGAACAAAATGTAAAGCCAGCCATGCATTGTTAATCATCCATCAC
  TTCCCATTTTATGGAATTGCTTTTAAAATACATTTGGCCTCTGCCCTTCAGAAGACTCGTTTTTAAGGTG
  GAAACTCCTGTGTCTGTGTATATTACAAGCCTACATGACACAGTTGGATTTATTCTGCCAAACCTGTGTA
  GGCATTTTATAAGCTACATGTTCTAATTTTTACCGATGTTAATTATTTTGACAAATATTTCATATATTTT
  CATTGAAATGCACAGATCTGCTTGATCAATTCCCTTGAATAGGGAAGTAACATTTGCCTTAAATTTTTTC
  GACCTCGTCTTTCTCCATATTGTCCTGCTCCCCTGTTTGACGACAGTGCATTTGCCTTGTCACCTGTGAG
  CTGGAGAGAACCCAGATGTTGTTTATTGAATCTACAACTCTGAAAGAGAAATCAATGAAGCAAGTACAAT
  GTTAACCCTAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_138319.2
• Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. The encoded protease is constitutively secreted into the extracellular matrix and expressed in many tissues, including neuroendocrine, liver, gut, and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression and left-right patterning. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2014]
• Locus ID: 5046