hnRNP K (HNRNPK) (NM_002140) Human 3' UTR Clone

CAT#: SC214044

3`UTR clone of heterogeneous nuclear ribonucleoprotein K (HNRNPK) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HNRNPK
• Synonyms: AUKS; CSBP; HNRPK; TUNP
• ACCN: NM_002140
• Insert Size: 1346 bp
• Sequence Data:
  Insert Sequence

  >SC214044 3'UTR clone of NM_002140
  The sequence shown below is from the reference sequence of NM_002140. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCAGAACAGTGTGAAGCAGTATGCAGATGTTGAAGGATTCTAATGCAAGATATTTTTTCTTTTTTATAGT
  GTGAAGCAGTATTCTGGAAAGTTTTTCTAAGACTAGTGAAGAACTGAAGGAGTCCTGCATCTTTTTTTTT
  TTATCTGCTTCTGTTTAAAAAGCCAACATTCCTCTGCTTCATAGGTGTTCTGCATTTGAGGTGTAGTGAA
  ATCTTTGCTGTTCACCAGATGTAATGTTTTAGTTCCTTACAAACAGGGTTGGGGGGGGGAAGGGCGTGCA
  AAAACTAACATTGAAATTTTGAAACAGCAGCAGAGTGAGTGGATTTTATTTTTCGTTATTGTTGGTGGTT
  TAAAAAATTCCCCCCATGTAATTATTGTGAACACCTTGCTTTGTGGTCACTGTAACATTTGGGGGGTGGG
  ACAGGGAGGAAAAGTAACAATAGTCCACATGTCCCTGGCATCTGTTCAGAGCAGTGTGCAGAATGTAATG
  CTCTTTTGTAAGAAACGTTTTATGATTTTTAAAATAAATTTAGTGAACCTATTTTTGGTGGTCATTTTTT
  TTTTAAGACAGTCATTTTAAAATGGTGGCTGAATTTCCCAACCCACCCCCAAACTAAACACTAAGTTTAA
  TTTTCAGCTCCTCTGTTGGACATATAAGTGCATCTCTTGTTGGACATAGGCAAAATAACTTGGCAAACTT
  AGTTCTGGTGATTTCTTGATGGTTTGGAAGTCTATTGCTGGGAAGAAATTCCATCATACATATTCATGCT
  TATAATAAGCTGGGGATTTTTTGTTTGTTTTTGCAAATGCTTGCCCCTACTTTTCAACAATTTTCTATGT
  TAGTTGTGAAGAACTAAGGTGGGGAGCAGTACTACAAGTTGAGTAATGGTATGAGTATATACCAGAATTC
  TGATTGGCAGCAAGTTTTATTAATCAGAATAACACTTGGTTATGGAAGTGACTAATGCTGAAAAAATTGA
  TTATTTTTATTAGATAATTTCTCACCTATAGACTTAAACTGTCAATTTGCTCTAGTGTCTTATTAGTTAA
  ACTTTGTAAAATATATATATACTTGTTTTTCCATTGTATGCAAATTGAAAGAAAAAGATGTACCATTTCT
  CTGTTGTATGTTGGATTATGTAGGAAATGTTTGTGTACAATTCAAAAAAAAAAAAGATGAAAAAAGTTCC
  TGTGGATGTTTTGTGTAGTATCTTGGCATTTGTATTGATAGTTAAAATTCACTTCCAAATAAATAAAACA
  CCCATGATGCTAGATTTGATGTGTGCCCGATTTGAACAAGGGTTGATTGACACCTGTAAAATTTGTTGAA
  ACGTTCCTCTTAAAAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002140.3
• Summary: 'This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]'
• Locus ID: 3190