HIF-1 alpha (HIF1A) (NM_181054) Human 3' UTR Clone

CAT#: SC214052

3`UTR clone of hypoxia inducible factor 1 alpha subunit (basic helix-loop-helix transcription factor) (HIF1A) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HIF1A
• Synonyms: bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
• ACCN: NM_181054
• Insert Size: 1363
• Sequence Data:
  Insert Sequence

  >SC214052 3'UTR clone of NM_181054
  The sequence shown below is from the reference sequence of NM_181054. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGAACATGATGGTTCACTTTTTCAAGCAGTAGGAATTATTTAGCATGTAGACTGCTGGGGCAATCAATGG
  ATGAAAGTGGATTACCACAGCTGACCAGTTATGATTGTGAAGTTAATGCTCCTATACAAGGCAGCAGAAA
  CCTACTGCAGGGTGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGAGCTTTTTCTTAATTTCATTCC
  TTTTTTTGGACACTGGTGGCTCATTACCTAAAGCAGTCTATTTATATTTTCTACATCTAATTTTAGAAGC
  CTGGCTACAATACTGCACAAACTTGGTTAGTTCAATTTTGATCCCCTTTCTACTTAATTTACATTAATGC
  TCTTTTTTAGTATGTTCTTTAATGCTGGATCACAGACAGCTCATTTTCTCAGTTTTTTGGTATTTAAACC
  ATTGCATTGCAGTAGCATCATTTTAAAAAATGCACCTTTTTATTTATTTATTTTTGGCTAGGGAGTTTAT
  CCCTTTTTCGAATTATTTTTAAGAAGATGCCAATATAATTTTTGTAAGAAGGCAGTAACCTTTCATCATG
  ATCATAGGCAGTTGAAAAATTTTTACACCTTTTTTTTCACATTTTACATAAATAATAATGCTTTGCCAGC
  AGTACGTGGTAGCCACAATTGCACAATATATTTTCTTAAAAAATACCAGCAGTTACTCATGGAATATATT
  CTGCGTTTATAAAACTAGTTTTTAAGAAGAAATTTTTTTTGGCCTATGAAATTGTTAAACCTGGAACATG
  ACATTGTTAATCATATAATAATGATTCTTAAATGCTGTATGGTTTATTATTTAAATGGGTAAAGCCATTT
  ACATAATATAGAAAGATATGCATATATCTAGAAGGTATGTGGCATTTATTTGGATAAAATTCTCAATTCA
  GAGAAATCATCTGATGTTTCTATAGTCACTTTGCCAGCTCAAAAGAAAACAATACCCTATGTAGTTGTGG
  AAGTTTATGCTAATATTGTGTAACTGATATTAAACCTAAATGTTCTGCCTACCCTGTTGGTATAAAGATA
  TTTTGAGCAGACTGTAAACAAGAAAAAAAAAATCATGCATTCTTAGCAAAATTGCCTAGTATGTTAATTT
  GCTCAAAATACAATGTTTGATTTTATGCACTTTGTCGCTATTAACATCCTTTTTTTCATGTAGATTTCAA
  TAATTGAGTAATTTTAGAAGCATTATTTTAGGAATATATAGTTGTCACAGTAAATATCTTGTTTTTTCTA
  TGTACATTGTACAAATTTTTCATTCCTTTTGCTCTTTGTGGTTGGATCTAACACTAACTGTATTGTTTTG
  TTACATCAAATAAACATCTTCTGTGGACCAGGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_181054.2
• Summary: This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
• Locus ID: 3091