ATF1 (NM_005171) Human 3' UTR Clone

CAT#: SC214060

3`UTR clone of activating transcription factor 1 (ATF1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ATF1
• Synonyms: EWS-ATF1; FUS/ATF-1; TREB36
• ACCN: NM_005171
• Insert Size: 1366
• Sequence Data:
  Insert Sequence

  >SC214060 3'UTR clone of NM_005171
  The sequence shown below is from the reference sequence of NM_005171. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAGTTAAAAACTTTGAAGGATCTTTATTCCAATAAAAGTGTTTGATTCCTAAGAAAGAAAATATTTTTGT
  GGACATGCATAAAAATTAAATGGATTTCCTAGTGGAGTTTTATAAATTAAAAGGTCAAAACTGAAGCTTT
  TTATTTAGGCTTTTCCAAATCAAGGATAAATATCTTACGCACGATATCTAGTGACAGAGGAGAAAGTGGA
  AAATGACCTCAAGGAAGCTACGGGCACAACTGGAAGCTTTGTAGAAATTAAACATATTCAAGGAGCAAGA
  AATGAACTTTCAGCAGTCTAAATTTTCTAAATAACCAATAGTTGCCAATCTAAAATGGCAGAGAAGATGA
  AATTTGATAAACTGAATTTTTTTTAAAAATCCATTTACCCTACAGGTTTGCATTTGTTTGCTGAAATTTA
  CCTTTTTTTAGTTATATATATGTGTGTGTGTGTGTGTAATTTCTGCCAATAAATTCTAAATTACAAAGGT
  AAGAGAAAACCTAGTACATTACTAAATATATAAAGTATATGTTCTGATTATGTATACTTGTTCTAGTGTC
  AAGTCTTTTTAAGTGGGTTTTTAAAAGTTTGTTATTGGACTTGAATGGATTTTTGAGACTAGGTTAATTA
  TTTTTGAGGTCTTATCCTAAAAGGCATCTAAGGTACATGAATGGAGTATGGTGATTTTATAACATTTTTT
  ATCAGAATGGAAAAAGAACTGTTTAAAAGTTTGATACTTTTAAATAGTTGGTTTTTTTGCTTACTCTGGT
  AATGATTTTCTACAAATACATAATAAATTGTTTTTTTGAGTCTATATTCTGTATGCAGTTGAATATCCAT
  TACTTATTCTGCTGTGCTTTAATAGAATGGAATGTTTACAGGCCCTTAAAATATTATTTTTAAAAAACCT
  TCTGAAGATACATACCAAAGTTTTTCCAAGAAGATTTTATAATCAATTTAATAATGTAAGGTTTATCAGA
  TTCTATAATAGTATAGTTATTAAGGCAATTTTATGTTAGAGACTATTTTGTAATGTAGTGAGTGGTACCT
  TTATAAGAAAAGTGACTGCCAATATATTTTTATAGCTGATCTTTATAAATTCTAATGTTGAGTTTTTAAT
  GATTATTTTAAATGTTTATATAGTTTAGTAAAATTTGCATCTCAAAGTATCATTTTTATATTATGGGACG
  TTTTCAGATTGGCTAATATTTGCATTGTAAATTTTGTATGCAGTTTATCTAAAATTCAAAAATACTGTCA
  GTACACCAGCGTTTAACATCTATATTCCAATTTGTATACAGTTTAAAATTGTACTGCAAAACTATTGTGT
  GCTCTTACACAGTATGCATCATATTGTTGTCTGTGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_005171.3
• Summary: This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010]
• Locus ID: 466