CD16 (FCGR3A) (NM_000569) Human 3' UTR Clone

CAT#: SC214083

3`UTR clone of Fc fragment of IgG low affinity IIIa receptor (CD16a) (FCGR3A) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: FCGR3A
• Synonyms: CD16; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20
• ACCN: NM_000569
• Insert Size: 1256 bp
• Sequence Data:
  Insert Sequence

  >SC214083 3'UTR clone of NM_000569
  The sequence shown below is from the reference sequence of NM_000569. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGGAGAAAGGACCCTCAAGACAAATGACCCCCATCCCATGGGGGTAATAAGAGCAGTAGCAGCAGCATCT
  CTGAACATTTCTCTGGATTTGCAACCCCATCATCCTCAGGCCTCTCTACAAGCAGCAGGAAACATAGAAC
  TCAGAGCCAGATCCCTTATCCAACTCTCGACTTTTCCTTGGTCTCCAGTGGAAGGGAAAAGCCCATGATC
  TTCAAGCAGGGAAGCCCCAGTGAGTAGCTGCATTCCTAGAAATTGAAGTTTCAGAGCTACACAAACACTT
  TTTCTGTCCCAACCGTTCCCTCACAGCAAAGCAACAATACAGGCTAGGGATGGTAATCCTTTAAACATAC
  AAAAATTGCTCGTGTTATAAATTACCCAGTTTAGAGGGGAAAAAAAAACAATTATTCCTAAATAAATGGA
  TAAGTAGAATTAATGGTTGAGGCAGGACCATACAGAGTGTGGGAACTGCTGGGGATCTAGGGAATTCAGT
  GGGACCAATGAAAGCATGGCTGAGAAATAGCAGGTAGTCCAGGATAGTCTAAGGGAGGTGTTCCCATCTG
  AGCCCAGAGATAAGGGTGTCTTCCTAGAACATTAGCCGTAGTGGAATTAACAGGAAATCATGAGGGTGAC
  GTAGAATTGAGTCTTCCAGGGGACTCTATCAGAACTGGACCATCTCCAAGTATATAACGATGAGTCCTCT
  TAATGCTAGGAGTAGAAAATGGTCCTAGGAAGGGGACTGAGGATTGCGGTGGGGGGTGGGGTGGAAAAGA
  AAGTACAGAACAAACCCTGTGTCACTGTCCCAAGTTGCTAAGTGAACAGAACTATCTCAGCATCAGAATG
  AGAAAGCCTGAGAAGAAAGAACCAACCACAAGCACACAGGAAGGAAAGCGCAGGAGGTGAAAATGCTTTC
  TTGGCCAGGGTAGTAAGAATTAGAGGTTAATGCAGGGACTGTAAAACCACCTTTTCTGCTTCAATATCTA
  ATTCCTGTGTAGCTTTGTTCATTGCATTTATTAAACAAATGTTGTATAACCAATACTAAATGTACTACTG
  AGCTTCGCTGAGTTAAGTTATGAAACTTTCAAATCCTTCATCATGTCAGTTCCAATGAGGTGGGGATGGA
  GAAGACAATTGTTGCTTATGAAAGAAAGCTTTAGCTGTCTCTGTTTTGTAAGCTTTAAGCGCAACATTTC
  TTGGTTCCAATAAAGCATTTTACAAGATCTTGCATGCTACTCTTAGATAGAAGATGGGAAAACCAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000569.6
• Summary: 'This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020]'
• Locus ID: 2214