PPT1 (NM_001142604) Human 3' UTR Clone

CAT#: SC214094

3`UTR clone of palmitoyl-protein thioesterase 1 (PPT1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PPT1
• Synonyms: CLN1; INCL; PPT
• ACCN: NM_001142604
• Insert Size: 1369 bp
• Sequence Data:
  Insert Sequence

  >SC214094 3'UTR clone of NM_001142604
  The sequence shown below is from the reference sequence of NM_001142604. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCCCACATCATACCATTCCTTGGATGAAACCCGTATAGTTCACAATAGAGCTCAGGGAGCCCCTAACTCT
  TCCAAACCACATGGGAGACAGTTTCCTTCATGCCCAAGCCTGAGCTCAGATCCAGCTTGCAACTAATCCT
  TCTATCATCTAACATGCCCTACTTGGAAAGATCTAAGATCTGAATCTTATCCTTTGCCATCTTCTGTTAC
  CATATGGTGTTGAATGCAAGTTTAATTACCATGGAGATTGTTTTACAAACTTTTGATGTGGTCAAGTTCA
  GTTTTAGAAAAGGGAGTCTGTTCCAGATCAGTGCCAGAACTGTGCCCAGGCCCAAAGGAGACAACTAACT
  AAAGTAGTGAGATAGATTCTAAGGGCAAACATTTTTCCAAGTCTTGCCATATTTCAAGCAAAGAGGTGCC
  CAGGCCTGAGGTACTCACATAAATGCTTTGTTTTGCTGGTGATTTAACCAGTGCTTGGAAAAATCTTGCT
  TGGCTATTTCTGCATCATTTCTTAAGGCTGCCTTCCTCTCTCAGTACGTTGCCCTCTGTGCTATCATCTT
  ATCATCAATTATTAGACAAATCCCACTGGCCTACAGTCTTGCTTCTGCAGCACCCACTTTGTCTCCTCAG
  GTAGTGATGAATTAGTTGCTGTCACAAAAGGAGGGAAGTAGCACCCAAATTAAGTTGCTTAAGAGAGGAA
  ATGTACATCTTGTATAACTTAGGGAGCGAAGAAAATGTAGGCGCGAAAGTGAAAAGTGAGGCAGCTAGTT
  CTTCCTATTCCATTCTCGACCAACCTGCCCTTTCTTAATATGACTAGTGGTCTTGATGCTAGAGTCAACT
  TACTCTGTTGCTGGCTTTAGCAGAGAATAGGAGGAACCATATGAAAAAGATCAGGCTTTCTGACTTCCAT
  CCCCAAAACACATTTACCAGCATACTCCAAACTGTTTCTGATGTGTTCCATGAGAAAAGGATTGTTTGCT
  CAAAAAGCTTGGAAAATACTACACACTCCCTTTCTCCTTCTGGAGATCAACCCACATTAGAGTGTCTAAG
  GACTCCTGAGAATTCCTGTTACAGTAAACAAAACTAACGTAATCTACCATTTCCTACACTATTTGAGCAT
  GGAAATCATAGTCCCCACTCTGTGAAAACTTAACGCTTTTTGGAAGACATTTCTGTAGCATGTCAGTTTG
  GAGAAATGATGAGCTACGCCTTGATGAAAGAACCGTGTTGGTGCTGCTAAGTTTAGCCATTATGGTTTTT
  CCTTTCTCTCTCTTAAGCCTTATTCTTCAACTAAAAGATGAGGATTAAGAGCAAGAAGTTGGGGGGGATG
  TGAAAATAATTTTATGAGGTTGTCTAAAATAAAGAGTAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001142604.1
• Summary: 'The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2008]'
• Locus ID: 5538