IRAK (IRAK1) (NM_001025243) Human 3' UTR Clone

CAT#: SC214195

3`UTR clone of interleukin-1 receptor-associated kinase 1 (IRAK1) transcript variant 3 for miRNA target validation

Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol IRAK1
Synonyms IRAK; pelle
ACCN NM_001025243
Insert Size 1363 bp
Sequence Data
>SC214195 3'UTR clone of NM_001025243
The sequence shown below is from the reference sequence of NM_001025243. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

GCCCGAAGAAAGTGATGAATTTCAGAGCTGATGTGTTCACCTGGGCAGATCCCCCAAATCCGGAAGTCAA
AGTTCTCATGGTCAGAAGTTCTCATGGTGCACGAGTCCTCAGCACTCTGCCGGCAGTGGGGGTGGGGGCC
CATGCCCGCGGGGGAGAGAAGGAGGTGGCCCTGCTGTTCTAGGCTCTGTGGGCATAGGCAGGCAGAGTGG
AACCCTGCCTCCATGCCAGCATCTGGGGGCAAGGAAGGCTGGCATCATCCAGTGAGGAGGCTGGCGCATG
TTGGGAGGCTGCTGGCTGCACAGACCCGTGAGGGGAGGAGAGGGGCTGCTGTGCAGGGGTGTGGAGTAGG
GAGCTGGCTCCCCTGAGAGCCATGCAGGGCGTCTGCAGCCCAGGCCTCTGGCAGCAGCTCTTTGCCCATC
TCTTTGGACAGTGGCCACCCTGCACAATGGGGCCGACGAGGCCTAGGGCCCTCCTACCTGCTTACAATTT
GGAAAAGTGTGGCCGGGTGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGAGGA
TCGCTGGAGCCCAGTAGGTCAAGACCAGCCAGGGCAACATGATGAGACCCTGTCTCTGCCAAAAAATTTT
TTAAACTATTAGCCTGGCGTGGTAGCGCACGCCTGTGGTCCCAGCTGCTGGGGAGGCTGAAGTAGGAGGA
TCATTTATGCTTGGGAGGTCGAGGCTGCAGTGAGTCATGATTGTATGACTGCACTCCAGCCTGGGTGACA
GAGCAAGACCCTGTTTCAAAAAGAAAAACCCTGGGAAAAGTGAAGTATGGCTGTAAGTCTCATGGTTCAG
TCCTAGCAAGAAGCGAGAATTCTGAGATCCTCCAGAAAGTCGAGCAGCACCCACCTCCAACCTCGGGCCA
GTGTCTTCAGGCTTTACTGGGGACCTGCGAGCTGGCCTAATGTGGTGGCCTGCAAGCCAGGCCATCCCTG
GGCGCCACAGACGAGCTCCGAGCCAGGTCAGGCTTCGGAGGCCACAAGCTCAGCCTCAGGCCCAGGCACT
GATTGTGGCAGAGGGGCCACTACCCAAGGTCTAGCTAGGCCCAAGACCTAGTTACCCAGACAGTGAGAAG
CCCCTGGAAGGCAGAAAAGTTGGGAGCATGGCAGACAGGGAAGGGAAACATTTTCAGGGAAAAGACATGT
ATCACATGTCTTCAGAAGCAAGTCAGGTTTCATGTAACCGAGTGTCCTCTTGCGTGTCCAAAAGTAGCCC
AGGGCTGTAGCACAGGCTTCACAGTGATTTTGTGTTCAGCCGTGAGTCACACTACATGCCCCCGTGAAGC
TGGGCATTGGTGACGTCCAGGTTGTCCTTGAGT

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Reference Data
RefSeq NM_001025243.1
Summary 'This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
Locus ID 3654

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.