IRAK (IRAK1) (NM_001569) Human 3' UTR Clone

CAT#: SC214198

3`UTR clone of interleukin-1 receptor-associated kinase 1 (IRAK1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: IRAK1
• Synonyms: IRAK; pelle
• ACCN: NM_001569
• Insert Size: 1363 bp
• Sequence Data:
  Insert Sequence

  >SC214198 3'UTR clone of NM_001569
  The sequence shown below is from the reference sequence of NM_001569. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCCCGAAGAAAGTGATGAATTTCAGAGCTGATGTGTTCACCTGGGCAGATCCCCCAAATCCGGAAGTCAA
  AGTTCTCATGGTCAGAAGTTCTCATGGTGCACGAGTCCTCAGCACTCTGCCGGCAGTGGGGGTGGGGGCC
  CATGCCCGCGGGGGAGAGAAGGAGGTGGCCCTGCTGTTCTAGGCTCTGTGGGCATAGGCAGGCAGAGTGG
  AACCCTGCCTCCATGCCAGCATCTGGGGGCAAGGAAGGCTGGCATCATCCAGTGAGGAGGCTGGCGCATG
  TTGGGAGGCTGCTGGCTGCACAGACCCGTGAGGGGAGGAGAGGGGCTGCTGTGCAGGGGTGTGGAGTAGG
  GAGCTGGCTCCCCTGAGAGCCATGCAGGGCGTCTGCAGCCCAGGCCTCTGGCAGCAGCTCTTTGCCCATC
  TCTTTGGACAGTGGCCACCCTGCACAATGGGGCCGACGAGGCCTAGGGCCCTCCTACCTGCTTACAATTT
  GGAAAAGTGTGGCCGGGTGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGAGGA
  TCGCTGGAGCCCAGTAGGTCAAGACCAGCCAGGGCAACATGATGAGACCCTGTCTCTGCCAAAAAATTTT
  TTAAACTATTAGCCTGGCGTGGTAGCGCACGCCTGTGGTCCCAGCTGCTGGGGAGGCTGAAGTAGGAGGA
  TCATTTATGCTTGGGAGGTCGAGGCTGCAGTGAGTCATGATTGTATGACTGCACTCCAGCCTGGGTGACA
  GAGCAAGACCCTGTTTCAAAAAGAAAAACCCTGGGAAAAGTGAAGTATGGCTGTAAGTCTCATGGTTCAG
  TCCTAGCAAGAAGCGAGAATTCTGAGATCCTCCAGAAAGTCGAGCAGCACCCACCTCCAACCTCGGGCCA
  GTGTCTTCAGGCTTTACTGGGGACCTGCGAGCTGGCCTAATGTGGTGGCCTGCAAGCCAGGCCATCCCTG
  GGCGCCACAGACGAGCTCCGAGCCAGGTCAGGCTTCGGAGGCCACAAGCTCAGCCTCAGGCCCAGGCACT
  GATTGTGGCAGAGGGGCCACTACCCAAGGTCTAGCTAGGCCCAAGACCTAGTTACCCAGACAGTGAGAAG
  CCCCTGGAAGGCAGAAAAGTTGGGAGCATGGCAGACAGGGAAGGGAAACATTTTCAGGGAAAAGACATGT
  ATCACATGTCTTCAGAAGCAAGTCAGGTTTCATGTAACCGAGTGTCCTCTTGCGTGTCCAAAAGTAGCCC
  AGGGCTGTAGCACAGGCTTCACAGTGATTTTGTGTTCAGCCGTGAGTCACACTACATGCCCCCGTGAAGC
  TGGGCATTGGTGACGTCCAGGTTGTCCTTGAGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001569.3
• Summary: 'This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 3654