BORIS (CTCFL) (NM_080618) Human 3' UTR Clone

CAT#: SC214474

3`UTR clone of CCCTC-binding factor (zinc finger protein)-like (CTCFL) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CTCFL
• Synonyms: BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
• ACCN: NM_080618
• Insert Size: 1397
• Sequence Data:
  Insert Sequence

  >SC214474 3'UTR clone of NM_080618
  The sequence shown below is from the reference sequence of NM_080618. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACCTGTGAAATGCTCCTCAACACGATGGATAAGTGAGAGGGATTCGGGTTGCGTGTTCACTGCCCCCAA
  TTCCTAAAGCAAGTTAGAAGTTTTTAGCATTTAAGGTGTGAAATGCTCCTCAACACGATGGATAAGTGAG
  AGAGAGTCAGGTTGCATGTTCACTGCCCCTAATTCCTAAAGCAAGTTAGAAATTTTTAGCATTTTCTTTG
  AAACAATTAAGTTCATGACAATGGATGACACAAGTTTGAGGTAGTGTCTAGAATTGTTCTCCTGTTTGTA
  GCTGGATATTTCAAAGAAACATTGCAGGTATTTTATAAAAGTTTTAAACCTTGAATGAGAGGGTAACACC
  TCAAACCTATGGATTCATTCACTTGATATTGGCAAGGTGGCCCACAATGAGTGAGTAGTGATTTTTGGAT
  ATTTCAAAATAGTCTAGACCAGCTAGTGCTTCCACAGTCAAAGCTGGACATTTTTATGTTGCATTATATA
  CACCCATGATATTTCTAATAATATATGGTTTTAAACATTAAAGACAAATGTTTTTATACAAATGAATTTT
  CTACAAAATTTAAAGCTACCATAATGCTTTTAATTAGTTCTAAATTCAACCAAAAAATGTTTTACTCTTA
  TAAAAAGGAAAACTGAGTAGGAAATGAAATACTAGATTAGACTAGAAAATAAGGAATAAATCGATTTTAC
  TTTGGTATAGGAGCAAGGTTCACCTTTAGATTTTTGTATTCTCTTTTAATTATGCTCCTTGGCAGGTATG
  AAATTGCCCTGGTTACATTCCATTATTGCTTATTAGTATTTCACTCCATAACCCTTTTTTCTGCTAAAAC
  TACTCTTTTTATATTTGTAAAATAATTGGCAGAGTGAGAAGAAACATAAAATCAGATAAGGCAAATGTGT
  ACCTGTAAGGAATTTGTACTTTTTCATAATGCCCAGTGATTAGTGAGTATTTCCCTTTTGCCAGTTGACA
  AGATTTTTCCACCCTCGAGCAGCGTGAGAGATGCCTCTTTAACACTTGAAATTCATTTCTATCTGGATAC
  AGAGGCAGATTTTTCTTCATTGCTTAGTTGAGCAGTTTGTTTTGCTGCCAACCTGTCTCCACCCCTGTAT
  TTCAAGATCATTGATAAGCCCTAAATTCAAATTCTTAAGATATGGACCTTTTATTGAAAATATCACAAGT
  TCAGAATCCCTATACAATGTGAATATGTGGAAATAATTTCCCAGCAGGAAGAGCATTATATTCTCTTTGT
  ACCAGCAAATTAATTTAACTCAACTCACATGAGATTTAAATTCTGTGGGCTGTAGTATGCCATCATTGTG
  ACTGAATTTGTGCAATGGTTTCTTAATTTTTTTACTGTTATTTAAAGATGTTTTACATAATTCAATA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_080618.2
• Summary: CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
• Locus ID: 140690